Overview

  • Product name

    Anti-TMS1/ASC antibody [EPR10402(B)]
    See all TMS1/ASC primary antibodies
  • Description

    Rabbit monoclonal [EPR10402(B)] to TMS1/ASC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMS1/ASC. The exact sequence is proprietary.

  • Positive control

    • THP1, MCF7, U937 cell lysates; THP1 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as TMS1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
    • Tissue specificity

      Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
    • Sequence similarities

      Contains 1 CARD domain.
      Contains 1 DAPIN domain.
    • Domain

      Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
    • Post-translational
      modifications

      Phosphorylated.
    • Cellular localization

      Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    Images

    • All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 22 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2:
      TMS1/ASC knockout HAP1 cell lysate (20 µg)
      Lane 3: U937 cell lysate (20 µg)
      Lane 4: THP1 cell lysate (20 µg)
      Lanes 1 - 4:
      Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
      Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
      Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)

      Lane 1 : THP1 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 22 kDa

    • Flow cytometric analysis of permeabilized THP1 cells labeling TMS1/ASC with unpurified ab151700 at 1/10 dilution (red). Rabbit IgG negative control (green).

    References

    ab151700 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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