Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10402(B)] to TMS1/ASC
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Product nameAnti-TMS1/ASC antibody [EPR10402(B)]
See all TMS1/ASC primary antibodies
DescriptionRabbit monoclonal [EPR10402(B)] to TMS1/ASC
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human TMS1/ASC. The exact sequence is proprietary.
- THP1, MCF7, U937 cell lysates; THP1 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as TMS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab151700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IP||1/10 - 1/100.|
FunctionPromotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificityWidely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 DAPIN domain.
DomainInteracts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
Cellular localizationCytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
- Information by UniProt
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Blocking and diluting buffer: 5% NFDM/TBST
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TMS1/ASC knockout HAP1 cell lysate (20 µg)
Lane 3: U937 cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)
Lane 1 : THP1 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
Flow cytometric analysis of permeabilized THP1 cells labeling TMS1/ASC with unpurified ab151700 at 1/10 dilution (red). Rabbit IgG negative control (green).
ab151700 has not yet been referenced specifically in any publications.