Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14066] to TMUB1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-TMUB1 antibody [EPR14066]
See all TMUB1 primary antibodies
DescriptionRabbit monoclonal [EPR14066] to TMUB1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human TMUB1 aa 50-200. The exact sequence is proprietary.
Database link: Q9BVT8
- Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation.
Sequence similaritiesContains 1 ubiquitin-like domain.
Cellular localizationMembrane. Cytoplasm. Nucleus. Predominantly nuclear during growth arrest (By similarity). Actively exported from the nucleus in dividing cells.
- Information by UniProt
- C7orf21 antibody
- Chromosome 7 Open Reading Frame 21 antibody
- Dendritic Cell-Derived Ubiquitin-Like Protein antibody
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling TMUB1 with ab180586 at 1/70 (pink). Control shown in green.
Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling TMUB1 with ab180586 at 1/500 (green). Dapi staining shown in blue.
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution
Lane 1 : Human fetal liver tissue lysate at 20 µg
Lane 2 : Human cerebellum tissue lysate at 20 µg
Lane 3 : HepG2 cell line lysate lysate at 20 µg
Lane 4 : HeLa cell line lysate at 10 µg
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible cleavage fragment)
ab180586 has been referenced in 1 publication.
- Fu H et al. Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes. Mol Med Rep 17:4337-4344 (2018). PubMed: 29344642