Overview

  • Product name

    Anti-TMUB1 antibody [EPR14066]
    See all TMUB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14066] to TMUB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TMUB1 aa 50-200. The exact sequence is proprietary.
    Database link: Q9BVT8

  • Positive control

    • Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14066
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/500.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation.
  • Sequence similarities

    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Membrane. Cytoplasm. Nucleus. Predominantly nuclear during growth arrest (By similarity). Actively exported from the nucleus in dividing cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • C7orf21 antibody
    • Chromosome 7 Open Reading Frame 21 antibody
    • Dendritic Cell-Derived Ubiquitin-Like Protein antibody
    • DULP antibody
    • Hepatocyte odd protein shuttling protein antibody
    • HOPS antibody
    • SB144 antibody
    • TMUB1 antibody
    • TMUB1_HUMAN antibody
    • Transmembrane and ubiquitin-like domain-containing protein 1 antibody
    • Ubiquitin-like protein DULP antibody
    • Ubiquitin-like protein SB144 antibody
    • UNQ763/PRO1555 antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling TMUB1 with ab180586 at 1/70 (pink). Control shown in green.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling TMUB1 with ab180586 at 1/500 (green). Dapi staining shown in blue.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100.

  • All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution

    Lane 1 : Human fetal liver tissue lysate at 20 µg
    Lane 2 : Human cerebellum tissue lysate at 20 µg
    Lane 3 : HepG2 cell line lysate lysate at 20 µg
    Lane 4 : HeLa cell line lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 24 kDa (possible cleavage fragment)

References

This product has been referenced in:

  • Fu H  et al. Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes. Mol Med Rep 17:4337-4344 (2018). Read more (PubMed: 29344642) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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