Recombinant Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14066] to TMUB1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TMUB1 antibody [EPR14066] - BSA and Azide free
See all TMUB1 primary antibodies -
Description
Rabbit monoclonal [EPR14066] to TMUB1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
-
General notes
ab250234 is the carrier-free version of ab180586.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14066 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa).
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa). |
Target
-
Function
May contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Membrane. Cytoplasm. Nucleus. Predominantly nuclear during growth arrest (By similarity). Actively exported from the nucleus in dividing cells. - Information by UniProt
-
Database links
- Entrez Gene: 83590 Human
- Entrez Gene: 64295 Mouse
- Entrez Gene: 362301 Rat
- Omim: 614792 Human
- SwissProt: Q9BVT8 Human
- SwissProt: Q9JMG3 Mouse
- SwissProt: Q53AQ4 Rat
- Unigene: 726215 Human
-
Alternative names
- C7orf21 antibody
- Chromosome 7 Open Reading Frame 21 antibody
- Dendritic Cell-Derived Ubiquitin-Like Protein antibody
see all
Images
-
All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TMUB1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human Cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab180586).
Lanes 1 - 4: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa cells in Western blot with loss of signal observed in TMUB1 knockout cell line ab278130 (knockout cell lysate ab278185). Wild-type HeLa and TMUB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab180586 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
This data was developed using ab180586, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab180586).
Lanes 1- 3: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa (Human epithelial line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution
Lane 1 : Human fetal liver tissue lysate at 20 µg
Lane 2 : Human cerebellum tissue lysate at 20 µg
Lane 3 : HepG2 cell line lysate lysate at 20 µg
Lane 4 : HeLa cell line lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible cleavage fragment)This data was developed using ab180586, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250234 has not yet been referenced specifically in any publications.