Product nameAnti-TMUB2 antibody [EPR13741]
See all TMUB2 primary antibodies
DescriptionRabbit monoclonal [EPR13741] to TMUB2
SpecificityBased on the sequence analysis, ab181183 recognizes 4 isoforms with predicted MW of 34KDa, 32KDa, 19KDa and 10KDa, respectively.
Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TMUB2 aa 50-200. The exact sequence is proprietary.
Database link: Q71RG4
- HeLa, HEK293, HepG2 and A431 cell lysates. HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/2000. Detects a band of approximately 10-34 kDa (predicted molecular weight: 34 kDa).|
|IP||1/30 - 1/50.|
Sequence similaritiesContains 1 ubiquitin-like domain.
- Information by UniProt
- FP2653 antibody
- MGC3123 antibody
- Tmub2 antibody
All lanes : Anti-TMUB2 antibody [EPR13741] (ab181183) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 10-34 kDa why is the actual band size different from the predicted?
Flow cytometry analysis of TMUB2 expression in HeLa cells using ab181183 at 1/150 dilution (red) and a rabbit IgG as negative control (green).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab181183 has not yet been referenced specifically in any publications.