Overview

  • Product name

  • Description

    Mouse polyclonal to TN-X
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human TN-X aa 1-673.

  • Positive control

    • TN-X transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight of full length protein: 464 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors.
    • Tissue specificity

      Highly expressed in fetal adrenal, in fetal testis, fetal smooth, striated and cardiac muscle. Isoform XB-short is only expressed in the adrenal gland.
    • Involvement in disease

      Tenascin-X deficiency (TNXD) [MIM:606408]: TNXD leads to an Ehlers-Danlos-like syndrome characterized by hyperextensible skin, hypermobile joints, and tissue fragility. Tenascin-X-deficient patients, however, lack atrophic scars, a major diagnostic criteria for classic Ehlers-Danlos. Delayed wound healing, which is also common in classic EDS, is only present in a subset of patients. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the tenascin family.
      Contains 19 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 32 fibronectin type-III domains.
    • Developmental stage

      Expression levels are lower in adults than in children.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • EDS3 antibody
      • Ehlers Danlos like syndrome antibody
      • Growth inhibiting protein 45 antibody
      • Hexabrachion like protein antibody
      • Hexabrachion-like protein antibody
      • HXBL antibody
      • NXB2 antibody
      • Tenascin X antibody
      • Tenascin X precursor antibody
      • Tenascin XB antibody
      • Tenascin XB1 antibody
      • Tenascin XB2 antibody
      • Tenascin-X antibody
      • TENX antibody
      • TENX_HUMAN antibody
      • TN X antibody
      • TN-X antibody
      • TNX antibody
      • TNXB antibody
      • TNXB1 antibody
      • TNXB2 antibody
      • TNXBS antibody
      • VUR8 antibody
      • XB antibody
      • XBS antibody
      see all

    Images

    • All lanes : Anti-TN-X antibody (ab67586) at 1/500 dilution

      Lane 1 : TN-X transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 464 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    References

    ab67586 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tendon)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    tendon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    zinc buffered formalin

    Abcam user community

    Verified customer

    Submitted Nov 03 2015

    Answer

    Thank you for your reply. I have issued your free of charge replacement with ab24718 on order ***.

    DISCOUNT CODE: ***
    Expiration date: December 6th, 2012

    I am very pleased to hear you would like to accept our offer and test ab81111 in WB on endogenous sample. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for endogenous samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for your reply.

    In order to qualify for the testing discount program, the antibody would need to be purchased for full price. I would be happy to send you this antibody as a free of charge replacement. Alternatively, If you would like to get the testing discount for ab81111 I can send you a replacement with a different antibody and a discount code for ab81111. Then if you purchase the antibody for full price, you can get a free antibody for submitting your Abreview using this antibody on endogenous samples in addition to the free of charge replacement for this case. Let me know how you would like to proceed and I will be happy to help you further.

    Read More

    Answer

    Thank you for your reply. I am sorry that this antibody is still not working in WB. Yes, we are happy to send you a free of charge replacement antibody. If you have an antibody to TNXB that is working for you, I can send you a replacement with an antibody to a different target or offer a credit or refund. Please let me know how you would like to proceed and I will be happy to help you further.

    Read More

    Answer

    Thank you for your reply. I hope that the 4-12% gel will help to visualize the larger molecular weight bands. If not, please let me know and I will be happy to offer you a free of charge replacement with an alternative antibody, or a credit or refund.

    Read More

    Answer

    Thank you for your reply. This antibody has not been validated for use on mouse samples. It is possible that it may cross-react, but we do not have any information about how it performs on mouse cells.

    In human samples, we observe an ˜80 kDa corresponding to the XB-short isoform of Tenascin-X. Because the mouse protein is not as well characterized, I was unable to find any information about whether this isoform is conserved in mouse or not.

    What percentage gel were you using? In order to detect the full length protein at ˜460 kDa, you would need to use a very low percentage gel with a slow wet transfer. It may also help to add 0.1% SDS to the transfer buffer and keep the methanol percentage to 10% or less. Even then, I would recommend validating your transfer with a Ponceau Sstain.

    Tenascin-X is also known to form a homotrimer and to interact with other large proteins. Since these complexes would be too large to detect in WB,it is very important that your samples are fully reduced and denatured. I would recommend running a positive control with a human skin sample to help determine whetherthe antibody is not reactive with the mouse protein or not working properly in general.

    I hope this helps, please let me know if you need any additional information or assistance and I will be happy to help you further.

    Read More

    Question
    Answer

    Thank you for contacting us. I am sorry that this antibody is not giving any signal in your experiment. I would be happy to help you further if you could please provide some additional information?

    1) What application were you working in (WB, IHC, etc)?

    2) What type of samples are you working with (species and tissue or cell type) and how were they prepared? What buffers were used for lysis, fixation, permeabilization, antigen retrieval, etc?

    3.) Which blocking agent was used, at what concentration and incubation time?

    4.) What was the primary antibody incubation time?

    5.) Which secondary antibody was used, at what concentration and incubation time?

    6.) What was the method of detection?

    Read More

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