Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)

Q&A (1)Specific References (1)

Overview

  • Product name
    Anti-TNF alpha Affibody® Molecule (Agarose)
    See all TNF alpha affibody® molecule
  • Conjugation
    Agarose
  • Conjugation notes
    This molecule is immobilized on agarose at the unique C-terminal cysteine.
  • Specificity
    ab31909 recognises TNF alpha.
  • Tested applications
    Suitable for: IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human TNF alpha. Recombinant protein produced in E. coli.

  • General notes

    ab31909 is a recombinant protein produced in E. coli.

    What are Affibody Molecules?
    Affibody® affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface- binding properties. The current Affibody® libraries contains billions of variants. In function, Affibody® molecules mimic antibodies, nature’s own binders to an infinite number of antigens. Compared to antibodies, the most striking dissimilarity of Affibody® molecules is the small size. Affibody® molecules have a molecular weight of 14 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of Affibody® molecules is similar to that of an antibody. The advantages of Affibody® molecules over antibodies are · their small size · the simple structure of the molecules · its robust physical properties · its ability to fold correctly intracellularly · the fast and cost-efficient production in bacteria · the possibility to produce Affibody® molecules through chemical synthesis · the possibility to couple Affibody® molecules in multimeric constructs

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: 0.328% Sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes
    ab31909 is >98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Affibody® molecule notes
    What are Affibody Molecules?
    Affibody® affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface- binding properties. The current Affibody® libraries contains billions of variants. In function, Affibody® molecules mimic antibodies, nature’s own binders to an infinite number of antigens. Compared to antibodies, the most striking dissimilarity of Affibody® molecules is the small size. Affibody® molecules have a molecular weight of 14 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of Affibody® molecules is similar to that of an antibody. The advantages of Affibody® molecules over antibodies are · their small size · the simple structure of the molecules · its robust physical properties · its ability to fold correctly intracellularly · the fast and cost-efficient production in bacteria · the possibility to produce Affibody® molecules through chemical synthesis · the possibility to couple Affibody® molecules in multimeric constructs
  • Research areas
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab31909 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at an assay dependent concentration.

    The molecule is immobilized on agarose at the unique C-terminal cysteine.

    This agarose immobilized Anti-TNF alpha Affibody® molecule is excellent for immunoprecipitation studies of TNF alpha in cell extracts or other solutions that contain TNF alpha proteins. Can also be used for affinity chromatography.

    Images

    • Immunoprecipitation - Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)
      Immunoprecipitation - Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)

      The agarose immobilized Anti-TNF alpha Affibody® molecule precipitates TNF-alpha protein from cell extracts derived from LPS stimulated but not from unstimulated THP-1 cells.

      RESULTS

      IMMUNOPRECIPITATION

      The human pro-monocytic cell line THP-1 was primed with PMA (200 nM) over night and stimulated with LPS (200 ng/ml) or with PBS (control) for 2 hours prior to termination of culture. Cell extracts prepared from LPS stimulated cultures and from control cells were incubated with agarose immobilized Anti-TNF-alpha Affibody® molecule for 2 hours. After incubation, the unbound proteins were washed away and the bound protein was eluted with SDS-PAGE separation and blotted onto a PVDF filter. The filter was stained with an antibody against full length TNF-alpha protein (25 kD).

      Figure 1a and 1b show that TNF-alpha was precipitated from LPS stimulated, but not from PBS stimulated THP-1 cell extract, using agarose immobilized Anti-TNF alpha Affibody® molecule. With increased amount of total protein, the TNF-alpha band became more intense. TNF-alpha was not detected in cell extracts prior to immunoprecipitation proving that an immunoprecipitation step is needed for TNF-alpha detection in this model system.

      These results show that the Anti-TNF alpha Affibody® molecule efficiently precipitates TNF-alpha proteins from a complex mix of proteins. When performing immunoprecipitation experiments with antibodies, there is often a problem with cross reactions between the enzyme conjugated second step reagent and the precipitating antibody. This type of cross reaction is avoided with an Affibody® molecule as the precipitating reagent.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab31909? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Q&A

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    Can you tell if this product contains cysteine blocked agarose?

    Thank you for your enquiry. I am sorry but we do not know this product contains cysteine blocked agarose. However, I can confirm that for immobilization of the Anti-TNF-alpha Affibody® Molecule on an agarose matrix we employ Pierce's SulfoLink Coupl...

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