Application
Other - Do not use
Sample
Human Purified protein (TNF alpha)
Secondary antibody
Secondary antibody
None used
Additional data
Notes
This antibody is used as capture antibody in sandwich lateral flow immunoassay experiments, while ab 222681 is used as detection antibody. TNF alpha (ab9642) is used as immunogen for these experiments.
Gold nanoparticles of size 40 nm were conjugated with ab 222681 with 100 ug/ml as concentration of antibody. BSA of concentration 0.5 wt% is used for blocking step. Volume of sample used for LFA experiments is 50 ul. OD of the conjugate is 5 and volume is 5 ul. Volume of running buffer is 45 ul containing 2% BSA and 1% tween-20.
The clinical range of detection for TNF alpha is 500 pg/ml to 0 pg/ml with LOD at 5 pg/ml.
By using this combination of antibody range of detection is observed from 5000 pg/ml to 100 pg/ml.
Abcam response
This is a non tested application (Lateral Flow Assay).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 26 2020