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Other Immunogen Type corresponding to Human TNF alpha. Human recombinant tumor necrosis factor of alpha type.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab215188 as a replacement.
Our Abpromise guarantee covers the use of ab8348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Neutralising||Use a concentration of 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TNF alpha (ab9635).
Use this antibody as Capture at 5µg/ml with ab9635 as Detection.
CON+ = Patient with increased TNF alpha levels on ELISA of muscle homogenate.
POS = Diseased patient with suspected increased TNF alpha levels in muscle.
ab8348 staining TNF alpha in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in a Tris buffer (pH 8). Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An undiluted HRP-conjugated Rabbit anti-mouse IgG polyclonal was used as the secondary antibody.
ICC/IF image of ab8348 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8348 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"