• Product name

  • Description

    Rabbit Polyclonal to TNF alpha
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human TNF alpha aa 1-233. The exact sequence is proprietary.
    Database link: P01375

  • Positive control

    • Human gastric carcinoma and HeLa xenograft tissues. Jurkat cells. Raji, 293T and TNF-transfected 293T whole cell lysates/extracts



Our Abpromise guarantee covers the use of ab183896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.


  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all


  • Anti-TNF alpha antibody (ab183896) at 1/1000 dilution + Raji whole cell lysate/extract at 30 µg

    Predicted band size: 26 kDa

  • All lanes : Anti-TNF alpha antibody (ab183896) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : TNF alpha-transfected 293T whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma, labeling TNF alpha using ab183896 at a 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded HeLa xenograft, labeling TNF alpha using ab183896 at a 1/500 dilution.

  • Immunofluorescence analysis of Jurkat cells fixed in 4% paraformaldehyde at RT for 15 minutes, labeling TNF alpha using ab183896 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.


This product has been referenced in:

  • Kim HA  et al. The Liver X Receptor Is Upregulated in Monocyte-Derived Macrophages and Modulates Inflammatory Cytokines Based on LXRa Polymorphism. Mediators Inflamm 2019:6217548 (2019). Read more (PubMed: 30944547) »
  • Giulbudagian M  et al. Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels. Theranostics 8:450-463 (2018). Read more (PubMed: 29290820) »
See all 3 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Baboon Tissue sections (brain hypothalamus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0
Yes - 0.4% triton x-100 in KPBS
brain hypothalamus
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C

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Submitted Aug 28 2018

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