Key features and details
- Rabbit Polyclonal to TNF alpha
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TNF alpha antibody
See all TNF alpha primary antibodies
DescriptionRabbit Polyclonal to TNF alpha
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human TNF alpha aa 1-233. The exact sequence is proprietary.
Database link: P01375
- Human gastric carcinoma and HeLa xenograft tissues. Jurkat cells. Raji, 293T and TNF-transfected 293T whole cell lysates/extracts
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 26 kDa.|
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
Anti-TNF alpha antibody (ab183896) at 1/1000 dilution + Raji whole cell lysate/extract at 30 µg
Predicted band size: 26 kDa
All lanes : Anti-TNF alpha antibody (ab183896) at 1/5000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : TNF alpha-transfected 293T whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma, labeling TNF alpha using ab183896 at a 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, labeling TNF alpha using ab183896 at a 1/500 dilution.
Immunofluorescence analysis of Jurkat cells fixed in 4% paraformaldehyde at RT for 15 minutes, labeling TNF alpha using ab183896 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
ab183896 has been referenced in 4 publications.
- Kempinska-Podhorodecka A et al. Oncomir MicroRNA-346 Is Upregulated in Colons of Patients With Primary Sclerosing Cholangitis. Clin Transl Gastroenterol 11:e00112 (2020). PubMed: 31972611
- Kim HA et al. The Liver X Receptor Is Upregulated in Monocyte-Derived Macrophages and Modulates Inflammatory Cytokines Based on LXRa Polymorphism. Mediators Inflamm 2019:6217548 (2019). PubMed: 30944547
- Giulbudagian M et al. Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels. Theranostics 8:450-463 (2018). PubMed: 29290820
- Firat T et al. 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-ß-D-glucopyranoside effect in liver regeneration. Histol Histopathol N/A:18056 (2018). PubMed: 30394506