Product nameAnti-TNF alpha antibody
See all TNF alpha primary antibodies
DescriptionRabbit polyclonal to TNF alpha
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-P, ELISA, RIA, Neutralisingmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse TNF alpha.
(Peptide available as
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis antibody is sterile filtered, and has been heated to 56°C for 30 minutes.
Endotoxin Content: <10 pg/µl by LAL method.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab34674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/400 - 1/800.|
|WB||1/400 - 1/800. Detects a band of approximately 17 kDa (predicted molecular weight: 26 kDa).Can be blocked with Synthetic Mouse TNF alpha protein (ab151311). Detects TNF alpha from supernatants or lysates of 2 x 106 endotoxin stimulated mouse peripheral blood mononuclear cells (PBMC).|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/2000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.|
|Neutralising||1/200. It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.|
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
Anti-TNF alpha antibody (ab34674) at 1/1000 dilution
HRP rabbit secondary antibody at 1/40000 dilution
Predicted band size: 26 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
ab34674 staining TNF alpha in immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA + 5% goat serum in PBS for 1 hour at 21°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 1 hour at 21°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
ab34674 staining TNF alpha in mouse knee joint sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10 minutes at room temperature followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.
This product has been referenced in:
- Qu W et al. Inhibition of colony-stimulating factor 1 receptor early in disease ameliorates motor deficits in SCA1 mice. J Neuroinflammation 14:107 (2017). Read more (PubMed: 28545543) »
- Rozas P et al. Targeted overexpression of tumor necrosis factor-a increases cyclin-dependent kinase 5 activity and TRPV1-dependent Ca2+ influx in trigeminal neurons. Pain 157:1346-62 (2016). Read more (PubMed: 26894912) »