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  1. Link

    tnf-alpha-antibody-ab9739.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-TNF alpha antibody (ab9739)

  • Datasheet
  • SDS
Reviews (11)Q&A (15)References (91)

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Western blot - Anti-TNF alpha antibody (ab9739)
  • Sandwich ELISA - Anti-TNF alpha antibody (ab9739)

Key features and details

  • Rabbit polyclonal to TNF alpha
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Recombinant fragment
  • Isotype: unknown

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Overview

  • Product name

    Anti-TNF alpha antibody
    See all TNF alpha primary antibodies
  • Description

    Rabbit polyclonal to TNF alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Mouse TNF alpha aa 80-235. E.coli-derived recombinant MurineTNF-alpha
    Database link: P06804

  • Positive control

    • Recombinant mouse TNF alpha protein (ab9740) can be used as a positive control in WB.
  • General notes

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Affibody® Molecule

    • Anti-TNF alpha Affibody® Molecule (ab31908)
  • Positive Controls

    • Recombinant mouse TNF alpha protein (Active) (ab9740)
  • Recombinant Protein

    • Recombinant human TNF alpha protein (Active) (ab259410)
    • Recombinant mouse TNF alpha protein (Active) (ab259411)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (8)
Use at an assay dependent concentration.

To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.

Sandwich ELISA
Use at an assay dependent concentration.

To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

Notes
WB
Use at an assay dependent concentration.

To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.

Sandwich ELISA
Use at an assay dependent concentration.

To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

Target

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Images

  • Western blot - Anti-TNF alpha antibody (ab9739)
    Western blot - Anti-TNF alpha antibody (ab9739)

    To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

    Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.

    Non-reducing conditions.

  • Sandwich ELISA - Anti-TNF alpha antibody (ab9739)
    Sandwich ELISA - Anti-TNF alpha antibody (ab9739)

    ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (91)

Publishing research using ab9739? Please let us know so that we can cite the reference in this datasheet.

ab9739 has been referenced in 91 publications.

  • Fernández-Albarral JA  et al. Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669765
  • Qi L  et al. microRNA-106b derived from endothelial cell-secreted extracellular vesicles prevents skin wound healing by inhibiting JMJD3 and RIPK3. J Cell Mol Med 25:4551-4561 (2021). PubMed: 33734576
  • Wei Q  et al. TNFa secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy. Acta Neuropathol Commun 9:67 (2021). PubMed: 33853689
  • Liu C  et al. Circular RNA circ_0128846 promotes the progression of osteoarthritis by regulating miR-127-5p/NAMPT axis. J Orthop Surg Res 16:307 (2021). PubMed: 33975612
  • Ji W  et al. Aldehyde Dehydrogenase 2 Protects Against Lipopolysaccharide-Induced Myocardial Injury by Suppressing Mitophagy. Front Pharmacol 12:641058 (2021). PubMed: 34025411
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 26 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: TBS/PEPSIN
Permeabilization
Yes - 0.03% Triton
Specification
Bone
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 24 2020

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice hepatocytes)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
80 µg
Specification
Mice hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Nov 20 2019

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
Mouse lung after 24-hour ischemia
Specification
Mouse lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 03 2017

Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (breast tumor)
Permeabilization
No
Specification
breast tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 15 2017

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Serum (Mouse Serum)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
Mouse Serum
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2014

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 08 2014

Question

I am looking for antibodies against neutrophils and TNF-alpha for Immunohistochemistry. I came across Anti-Neutrophil antibody (ab2557) and Anti-TNF alpha antibody(ab9739) that I can use for staining. But, I few questions regarding these antibodies,



1. Do these antibodies work on tissue sections of knee joint?

2. Knee joints are 4% formaldehyde fixed and paraffin embedded sections. Before embedding, knee joints are decalcified with 20% formic acid + Sodium citrate for 3 days. Do these antibodies work on this particular treated sections?

Read More

Abcam community

Verified customer

Asked on Feb 04 2013

Answer

Thank you for contacting us.

Unfortunately, we cannot test every organ or tissue when we establish a product.However, if the knee joints were decalcified properly and the species is tested, I don't see any problems why these antibodies shouldn't work in these sections.

Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 04 2013

Question

I am sending this e-mail to ask your help with these positive controls of the following antibodies:

Antibody Anti-IL6 (ab9324)

Antibody Anti-TNF Alfa (ab9739)

Antibody Anti-Interferon Gama (ab9657)

Read More

Abcam community

Verified customer

Asked on Jan 09 2013

Answer

All of these antibodies recognise cytokines which are present in inflammed tissue therefore any inflammed tissue would be a good positive control.

In our catalogue, we have several cancer slides which would be appropriate:
tonsil: (ab5146)
breast (ab4701)
pancreatic (ab5036)
stomach (ab5115)
colon (ab4702)

Alternatively, spleen tissues seem to be a very good positive control for these cytokines and are used on several of our other datasheets.

I would suggest using ab4372 if you are working on human samples, ab4613 if you are working on mouse samples or ab4624 if you are working on rat samples.

Read More

Abcam Scientific Support

Answered on Jan 09 2013

Question

Könnten Sie mir ein weiteres Angebot für Westernblott machen.Ich soll
TNFa, IL-6, IL-10 und HO1 von murinen Proben von ca 300 Proben
blotten. Haben Sie die dazu nötigen primären AK im Angebot? Welche
Sekundär AK empfehlen Sie uns und was kosten das?
Vielen Dank im voraus!

Read More

Abcam community

Verified customer

Asked on Nov 13 2012

Answer

Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nun damit verarbeitet bekommen kann ich leider nicht versprechen, da die Antikörper ja auch in den meisten Fällen ein paar Optimierungsexperimente benötigen.

https://www.abcam.com/TNF-alpha-antibody-ab9739.html (or use the following: https://www.abcam.com/TNF-alpha-antibody-ab9739.html).

https://www.abcam.com/IL-10-antibody-ab9969.html (or use the following: https://www.abcam.com/IL-10-antibody-ab9969.html).

https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html (or use the following: https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html).

https://www.abcam.com/IL-6-antibody-ab83339.html (or use the following: https://www.abcam.com/IL-6-antibody-ab83339.html).


Als Sekundär-AK würde ich Ihnen den ab97069 Goat anti-Rabbit IgG H&L (HRP) secondary antibody empfehlen, da dieser bis zu einer Verdünnung von 1/50.000 eingestzt werden kann.



https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html).


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere technische Fragen haben. Falls Sie die Produkte Bestellen möchten oder mehr Informationen zB bezüglich des Preises wünschen, wenden Sie sich einfach an unseren Kundendienst.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Nov 14 2012

1-10 of 26 Abreviews or Q&A

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