• Product name

  • Description

    Rabbit polyclonal to TNF alpha
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, Neutralising, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Recombinant fragment corresponding to Rat TNF alpha aa 80-235. E.coli-derived Recombinant Rat TNF-alpha
    Database link: P16599

  • Positive control

    • Recombinant rat TNF alpha protein (ab9756) can be used as a positive control in WB. ICC/IF: PC12 cells.



Our Abpromise guarantee covers the use of ab9755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect rTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA Use a concentration of 0.5 µg/ml.

To detect rTNF-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.

Neutralising Use a concentration of 0.145 - 0.165 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of rTNF-alpha (2.0 ng/ml), a concentration of 0.145 - 0.165 µg/ml of this antibody is required

ICC/IF Use a concentration of 5 µg/ml.


  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all


  • ICC/IF image of ab9755 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9755, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab9755, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • O'Sullivan SJ  et al. Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis. Front Neurosci 13:665 (2019). Read more (PubMed: 31333398) »
  • Xie J  et al. MicroRNA-146a improves sepsis-induced cardiomyopathy by regulating the TLR-4/NF-?B signaling pathway. Exp Ther Med 18:779-785 (2019). Read more (PubMed: 31281454) »
See all 16 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


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Thank you for your enquiry. I have read the details that you have submitted on behalf of your customer and I have a few comments. According to the EXPASY database TNF alpha is mainly secreted by macrophages. As I understand the heart does have associated macrophages. Please can you confirm that your customer is observing non-specific bands that do not include the actual band. The predicted molecular weight of this protein is 25.8KDa. However, the actual molecular weight as determined by western blot may differ from this, and I was wondering whether this might be the band migrating at 21 or 31 KDa. I would not recommend loading 150ug onto a western blot as this is definitely excessive. I would recommend a mass up to 50ug. However, I do understand that modern gel apparatus do tolerate this level of protein. With regards the protocol employed by your customer I would recommend that they perform an overnight incubation at 4oC using this antiserum at a 1:1000 (or less) dilution. This can improve the specificity of the result. I would also recommend the use of 5% BSA as this has been shown to improve the specificity of immunoblotting results. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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Thank you for your enquiry. According to the “Swiss Prot” reference for rat the predicted molecular weight is 28KDa so it is likely that the band that you are observing at 25KDa is the target. Have you used the recombinant form of the target as a positive control? The immunogen used to raise this product is a recombinant protein and currently all the data that we have relates to the detection of recombinants in Western blotting. We do not have any data relating to the specificity of this product, it has only been tested on the recombinant. Since the product is a polyclonal it will recognise several epitopes and so it would not be possible to predict whether the exact epitope that the Ab recognises is conserved in TNF-beta and TNFR2.

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All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). According to the datasheet, this antibody has not been tested for IHC application.

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