• Product name
    Anti-TNF alpha antibody [F6C5] (HRP)
    See all TNF alpha primary antibodies
  • Description
    Mouse monoclonal [F6C5] to TNF alpha (HRP)
  • Host species
  • Conjugation
  • Specificity
    This antibody demonstrates human TNF-alpha
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant tumor necrosis factor of alpha type (TNF alpha).



Our Abpromise guarantee covers the use of ab24473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [2C8] to TNF alpha (ab8348). Use as Detection antibody in conjugation with recommended pair.


  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all


This product has been referenced in:
  • Chiu WC  et al. Humanization and Characterization of an Anti-Human TNF-a Murine Monoclonal Antibody. PLoS One 6:e16373 (2011). ELISA ; Human . Read more (PubMed: 21305012) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


We recommend determining the optimal concentration by trying range of dilutions however for a start you can try concentration anywhere between 0.1-1ug/ml.

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Unfortunately, the ab24473 has not been tested for endotoxin level. Generally, it is not usual to determine the endotoxin levels of research grade antibodies. I am sorry for this inconvenience.

However, we have tested MAb F6C5 only in IHC and ELISA and we know that in sandwich ELISA it is very good as detection MAb together with MAb 2C8 as capture MAb.

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Thank you for contacting us.

The molecular weight of the monoclonal antibody ab24473 is about 150 kDa.

I hope this helps. Please let me know if I can help you further.

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Thank you for contacting us.

We have in our catalogue the following pairs of antibodies that have been tested for the detection of Human TNF-alpha in sELISA :

Anti-TNF alpha antibody [2C8] (https://www.abcam.com/ab8348) as capture + Rabbit polyclonal to TNF alpha (https://www.abcam.com/ab9635) as detection. Please note that these 2 antibodies are unconjugated.
Anti-TNF alpha antibody [2C8] (https://www.abcam.com/ab8348) as capture + Anti-TNF alpha antibody [F6C5 ] (HRP) (https://www.abcam.com/ab24473) as detection.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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