APC Anti-TNF alpha antibody [MAb11] (ab269282)
Key features and details
- APC Mouse monoclonal [MAb11] to TNF alpha
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-TNF alpha antibody [MAb11]
See all TNF alpha primary antibodies -
Description
APC Mouse monoclonal [MAb11] to TNF alpha -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
Recognizes human 17-26 kDa cytokine TNF-alpha.
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Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to TNF alpha. Recombinant human TNF-alpha
Database link: P01375 -
Positive control
- Flow Cyt (Intra): Human activated peripheral blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
MAb11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use 10µl for 106 cells.
Or 100 µL of whole blood. |
Notes |
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Flow Cyt (Intra)
Use 10µl for 106 cells. Or 100 µL of whole blood. |
Target
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7124 Human
- Omim: 191160 Human
- SwissProt: P01375 Human
- Unigene: 241570 Human
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Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab269282 has been referenced in 1 publication.
- Wang Q et al. Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-kB Signaling Pathway. Evid Based Complement Alternat Med 2021:8868527 (2021). PubMed: 33505510