Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free (ab34719)

Reviews (1)Q&A (2)References (5)

Key features and details

  • Rat monoclonal [MP6-XT22] to TNF alpha - BSA and Azide free
  • Suitable for: ELISA, IHC-Fr, Flow Cyt, Functional Studies
  • Reacts with: Mouse
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-TNF alpha antibody [EPR21753-109] (ab205587)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free
    See all TNF alpha primary antibodies

  • Description

    Rat monoclonal [MP6-XT22] to TNF alpha - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: ELISA, IHC-Fr, Flow Cyt, Functional Studiesmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein corresponding to Mouse TNF alpha. Recombinant murine TNF alpha expressed in E. coli.
    Database link: P06804

  • General notes


    This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilisation is required for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilisation is required for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.

Target

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    References (5)

    Publishing research using ab34719? Please let us know so that we can cite the reference in this datasheet.

    ab34719 has been referenced in 5 publications.

    • Zhang Z  et al. Design of a biofluid-absorbing bioactive sandwich-structured Zn-Si bioceramic composite wound dressing for hair follicle regeneration and skin burn wound healing. Bioact Mater 6:1910-1920 (2021). PubMed: 33364530
    • Kumstel S  et al. MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases. Sci Rep 10:16931 (2020). PubMed: 33037288
    • Brings S  et al. Diabetes-induced alterations in tissue collagen and carboxymethyllysine in rat kidneys: Association with increased collagen-degrading proteinases and amelioration by Cu(II)-selective chelation. Biochim Biophys Acta 1852:1610-8 (2015). PubMed: 25900786
    • Kimura N  et al. Injury and subsequent regeneration of muscles for activation of local innate immunity to facilitate the development and relapse of autoimmune myositis in C57BL/6 mice. Arthritis Rheumatol 67:1107-16 (2015). IHC-Fr ; Mouse . PubMed: 25580817
    • Scanga CA  et al. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun 67:4531-8 (1999). PubMed: 10456896

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry - Free Floating abreview for Anti-TNF alpha antibody [MP6-XT22]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Coronal brain slices)
    Specification
    Coronal brain slices
    Read More

    Miss. Ronit Shapira

    Verified customer

    Submitted Mar 23 2016

    Question

    Inquiry: Hi, Can you tell me if this antibody works on both PFA fixed and methanol fixed cells? Thanks,

    Read More
    Answer

    Thank you for your enquiry and interest.

    As the datasheet indicates this product has been characterized on IHC-Fr (frozen section for immunohistochemistry) but NOT on IHC-P (formalin-fixed paraffin-embedded sections). The samples can be fixed with methanol, acetone or ethanol. We do not have any data yet if ab34719 is suitable for formalin or PFA-fixed tissue sections.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Hello,   I am looking for injectable form of murine TNF-alpha antibody. Could you give me some advice whether I can use MP6-XT22 (ID: ab34719) for that purpose? And if yes, please advice on your recommending dosage.   Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. I can confirm that this antibody has been tested and characterized for in "in vitro" functional assay using cultured cells. I am very sorry to inform you that as the disclaimer states on the bottom of each of our datasheets: "all our products are for research use only and are not intended for diagnostic or therapeutic use". This means that we do not recommend our antibodies for "in vivo" studies and experiments; or for medical application. If you need any further assistance in the future, please do not hesitate to contact me.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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