Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free (ab34719)


  • Product name
    Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free
    See all TNF alpha primary antibodies
  • Description
    Rat monoclonal [MP6-XT22] to TNF alpha - BSA and Azide free
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, Functional Studies, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse TNF alpha. Recombinant murine TNF alpha expressed in E. coli.
    Database link: P06804

  • General notes

    This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.



Our Abpromise guarantee covers the use of ab34719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ELISA Use a concentration of 1 - 10 µg/ml.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.





ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all


This product has been referenced in:
  • Brings S  et al. Diabetes-induced alterations in tissue collagen and carboxymethyllysine in rat kidneys: Association with increased collagen-degrading proteinases and amelioration by Cu(II)-selective chelation. Biochim Biophys Acta 1852:1610-8 (2015). Read more (PubMed: 25900786) »
  • Kimura N  et al. Injury and subsequent regeneration of muscles for activation of local innate immunity to facilitate the development and relapse of autoimmune myositis in C57BL/6 mice. Arthritis Rheumatol 67:1107-16 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25580817) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry free floating
Mouse Tissue sections (Coronal brain slices)
Coronal brain slices

Miss. Ronit Shapira

Verified customer

Submitted Mar 23 2016


Thank you for your enquiry and interest.

As the datasheet indicates this product has been characterized on IHC-Fr (frozen section for immunohistochemistry) but NOT on IHC-P (formalin-fixed paraffin-embedded sections). The samples can be fixed with methanol, acetone or ethanol. We do not have any data yet if ab34719 is suitable for formalin or PFA-fixed tissue sections.

If you need any further assistance in the future, please do not hesitate to contact me.

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Thank you for your enquiry and your interest in our products. I can confirm that this antibody has been tested and characterized for in "in vitro" functional assay using cultured cells. I am very sorry to inform you that as the disclaimer states on the bottom of each of our datasheets: "all our products are for research use only and are not intended for diagnostic or therapeutic use". This means that we do not recommend our antibodies for "in vivo" studies and experiments; or for medical application. If you need any further assistance in the future, please do not hesitate to contact me.

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