Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free (ab34719)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free
See all TNF alpha primary antibodies -
Description
Rat monoclonal [MP6-XT22] to TNF alpha - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, Functional Studies, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein corresponding to Mouse TNF alpha. Recombinant murine TNF alpha expressed in E. coli.
Database link: P06804 -
General notes
This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified from tissue culture supernatant. -
Primary antibody notes
This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha. -
Clonality
Monoclonal -
Clone number
MP6-XT22 -
Isotype
IgG1 -
Research areas
Associated products
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Affibody® Molecule
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. | |
| ELISA | Use a concentration of 1 - 10 µg/ml. | |
| Flow Cyt | 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21926 Mouse
- SwissProt: P06804 Mouse
- Unigene: 1293 Mouse
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Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Brings S et al. Diabetes-induced alterations in tissue collagen and carboxymethyllysine in rat kidneys: Association with increased collagen-degrading proteinases and amelioration by Cu(II)-selective chelation. Biochim Biophys Acta 1852:1610-8 (2015). Read more (PubMed: 25900786) »
- Kimura N et al. Injury and subsequent regeneration of muscles for activation of local innate immunity to facilitate the development and relapse of autoimmune myositis in C57BL/6 mice. Arthritis Rheumatol 67:1107-16 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25580817) »
