Overview

  • Product name
    Anti-TNF alpha antibody [MP6-XT3]
    See all TNF alpha primary antibodies
  • Description
    Rat Monoclonal [MP6-XT3] to TNF alpha
  • Host species
    Rat
  • Specificity
    ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
  • Tested applications
    Suitable for: WB, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Mouse TNF alpha. Expressed in E. coli.
    Database link: P06804

  • General notes
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. This antibody recognises the 17 kDa soluble form of TNF alpha.
Flow Cyt Use at an assay dependent concentration.

Membrane permeabilization is required for this application

 

 

 

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ELISA Use a concentration of 5 µg/ml.

Target

  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Images

  • Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.

References

This product has been referenced in:
  • Yu B  et al. Morroniside on anti-inflammation activities in rats following acute myocardial infarction. Korean J Physiol Pharmacol 22:17-21 (2018). Read more (PubMed: 29302208) »
  • Patki G  et al. Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats. PLoS One 10:e0117498 (2015). WB ; Rat . Read more (PubMed: 25793256) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you very much for your inquiry. Reviewing the datasheets for these antibody I am confident that a single ELISPOT should give good results with this combination of antibodies. But unfortunately, these antibodies have not been tested in ELISPOT (only ELISA) and are therefore not covered by our Abpromise guarantee. I do not have experience with double ELISPOTs and therefore do not want to make a prediction. You might be eligible for our testing discount program if you would also perform a single ELISPOT with these antibodies. Please let me know if you are interested in this offer. I wish you good luck for your experiments.

Read More

Answer

Thank you for your email. Our source for this antibody tested ab11564 in Western blotting with recombinant TNF alpha. Conditions were standard. To use this antibody in Western blotting, I would recommend referring to our general Western blot protocol which can be found in the protocol section of the online datasheet. I would suggest using ab11564 at a 1:1000 dilution, and ab6734 at a 1:5000 dilution. I conferred with our source for ab11564 and it sounds like you are detecting the membrane bound form of TNF alpha (the 26 kDa integral membrane protein). If you have any additional questions, please contact us again.

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Question
Answer

This antibody recognises the 17 kDa soluble form of TNF alpha. TNF alpha forms dimers and trimers, and in addition is found as a 26 kDa integral membrane protein. I am not able to comment on the ability of this antibody to recognise the membrane bound form but I can tell you that we also see a trimer of the soluble form in WBs (running at around 50 kDa). If you have any additional questions, please contact us again.

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Answer

Thank you for your enquiry and for your patience. All the information that we have regarding these two antibodies is loacted on the online datasheets. To our knowledge, reactivity with TNF beta has still not yet been tested. Mature TNF-beta and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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