Overview

  • Product name

    Anti-TNF Receptor I antibody
    See all TNF Receptor I primary antibodies
  • Description

    Goat polyclonal to TNF Receptor I
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cat, Dog, Pig, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human TNF Receptor I aa 381-394 (internal sequence) (Cysteine residue).
    Sequence:

    SDHEIDRLELQNGR


    Database link: P19438

  • Positive control

    • WB: Human Lung lysate, A549, HepG2, K562

Properties

Applications

Our Abpromise guarantee covers the use of ab134562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function

    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease

    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain

    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications

    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

Images

  • All lanes : Anti-TNF Receptor I antibody (ab134562) at 0.5 µg/ml

    Lane 1 : A549 (Human lung carcinoma)
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma)
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-TNF Receptor I antibody (ab134562) at 0.3 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

References

ab134562 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134562.
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