Key features and details
- Goat polyclonal to TNF Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies
DescriptionGoat polyclonal to TNF Receptor I
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cat, Dog, Pig, Cynomolgus monkey, Rhesus monkey
- WB: Human Lung lysate, A549, HepG2, K562
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab134562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 50 kDa.
1 hour primary incubation is recommended for this product.
FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
- CD120a antibody
- FPF antibody
- MGC19588 antibody
All lanes : Anti-TNF Receptor I antibody (ab134562) at 0.5 µg/ml
Lane 1 : A549 (Human lung carcinoma)
Lane 2 : HepG2 (Human liver hepatocellular carcinoma)
Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-TNF Receptor I antibody (ab134562) at 0.3 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
ab134562 has not yet been referenced specifically in any publications.