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    tnf-receptor-i-antibody-ab19139.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-TNF Receptor I antibody (ab19139)

  • Datasheet
  • SDS
Reviews (18)Q&A (19)References (91)

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Western blot - Anti-TNF Receptor I antibody (ab19139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)

Key features and details

  • Rabbit polyclonal to TNF Receptor I
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TNF Receptor I antibody
    See all TNF Receptor I primary antibodies
  • Description

    Rabbit polyclonal to TNF Receptor I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.

  • Positive control

    • WB: Heat-shocked HeLa cell lysate, Jurkat cell lysate. IHC-P: Human spleen

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Necrosis Factors
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • TNF ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Cancer
    • Cell Death
    • Necroptosis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human TNF Receptor I protein (Active) (ab224647)

Applications

Our Abpromise guarantee covers the use of ab19139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function

    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease

    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain

    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications

    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Target information above from: UniProt accession P19438 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7132 Human
    • Omim: 191190 Human
    • SwissProt: P19438 Human
    • Unigene: 279594 Human
    • Unigene: 713833 Human
    • Alternative names

      • CD120a antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55-R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    Images

    • Western blot - Anti-TNF Receptor I antibody (ab19139)
      Western blot - Anti-TNF Receptor I antibody (ab19139)
      All lanes : Anti-TNF Receptor I antibody (ab19139) at 1 µg/ml

      Lane 1 : Heat-shocked HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)
      Immunohistochemistry Formalin/PFA-fixed paraffin-embedded human spleen sections labeling TNF Receptor I with ab19139 at 10ug/mL.

       

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (91)

    Publishing research using ab19139? Please let us know so that we can cite the reference in this datasheet.

    ab19139 has been referenced in 91 publications.

    • Zhang Y  et al. EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-a on osteogenic differentiation. BMC Mol Cell Biol 21:29 (2020). PubMed: 32299362
    • Wang H  et al. Astragaloside III activates TACE/ADAM17-dependent anti-inflammatory and growth factor signaling in endothelial cells in a p38-dependent fashion. Phytother Res 34:1096-1107 (2020). PubMed: 32197276
    • Hwang HS  et al. Involvement of the TNF-a Pathway in TKI Resistance and Suggestion of TNFR1 as a Predictive Biomarker for TKI Responsiveness in Clear Cell Renal Cell Carcinoma. J Korean Med Sci 35:e31 (2020). PubMed: 32030920
    • Vial J  et al. The Ectodysplasin receptor EDAR acts as a tumor suppressor in melanoma by conditionally inducing cell death. Cell Death Differ 26:443-454 (2019). PubMed: 29855541
    • Vickman RE  et al. Cholesterol Sulfotransferase SULT2B1b Modulates Sensitivity to Death Receptor Ligand TNFa in Castration-Resistant Prostate Cancer. Mol Cancer Res 17:1253-1263 (2019). PubMed: 30824526
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 37 Abreviews or Q&A

    Question

    Inquiry: I got a new vial of TNFR antibody (Lot: ****). However,we got no signal with it. We ran: 30ug total mouse liver lysate (120V 2hrs and transferring 250 mA 2hrs, blocking in 5% non-fat milk 1hrs RT, primary 1:1000 in 1 % milk at 4 degree overnight, wash 3 X 5min in TBST 2nd anti rabbit from sigma 1hr at RT, wash 3 X 5min in TBST detected with pierce ECL When I stripped the same membrane and used the old vial antibody (abcam, cat: a b19139, lot: ****), I got the proper band. Could you help me to see what is wrong with the vial (lot: ****)?

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2012

    Answer

    Thank you for contacting Abcam.

    I am sorry that there seems to be a problem with the current lot of ab19139 that you have. If you can provide me with the Abcam order number for this vial of ab19139, I will be happy to send a replacement vial out to you.

    Looking forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on May 07 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF Receptor I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adult Tenocytes)
    Permeabilization
    Yes - 0.1% Triton-X-100
    Specification
    Adult Tenocytes
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Emily Smith

    Verified customer

    Submitted Nov 30 2020

    Western blot abreview for Anti-TNF Receptor I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PC3 Prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    PC3 Prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jun 13 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF Receptor I antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C42B Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 11 2019

    Western blot abreview for Anti-TNF Receptor I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Intestinal epithelial cell)
    Gel Running Conditions
    Reduced Denaturing (6-15% gradient gel)
    Loading amount
    20 µg
    Specification
    Intestinal epithelial cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 01 2016

    Western blot abreview for Anti-TNF Receptor I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    100 ng/ml LPS
    Specification
    mouse hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF Receptor I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Sample
    Mouse Cell (hepatocytes)
    Specification
    hepatocytes
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 19 2014

    Question

    How was pig reactivity determined?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 15 2014

    Answer

    Our guarantee (www.abcam.com/abpromise) will cover pig reactivity for the TNFR1 antibody ab19139, but we do not have the data that demonstrated cross-reactivity. A customer several years ago used this antibody to stain sections of pig placenta and reported positive results but we regrettably did not obtain the protocol or data.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jan 15 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF Receptor I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW264.7)
    Specification
    RAW264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton + 2% BSA in PBS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Apr 26 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF Receptor I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 Standard
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 17 2012

    1-10 of 37 Abreviews or Q&A

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