Anti-TNF Receptor I antibody (ab19139)
Key features and details
- Rabbit polyclonal to TNF Receptor I
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies -
Description
Rabbit polyclonal to TNF Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.
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Positive control
- WB: Heat-shocked HeLa cell lysate, Jurkat cell lysate. IHC-P: Human spleen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab19139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
Target
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
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Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
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Western blot - Anti-TNF Receptor I antibody (ab19139)All lanes : Anti-TNF Receptor I antibody (ab19139) at 1 µg/ml
Lane 1 : Heat-shocked HeLa cell lysate
Lane 2 : Jurkat cell lysate
Predicted band size: 50 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)Immunohistochemistry Formalin/PFA-fixed paraffin-embedded human spleen sections labeling TNF Receptor I with ab19139 at 10ug/mL.
Protocols
References (91)
ab19139 has been referenced in 91 publications.
- Zhang Y et al. EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-a on osteogenic differentiation. BMC Mol Cell Biol 21:29 (2020). PubMed: 32299362
- Wang H et al. Astragaloside III activates TACE/ADAM17-dependent anti-inflammatory and growth factor signaling in endothelial cells in a p38-dependent fashion. Phytother Res 34:1096-1107 (2020). PubMed: 32197276
- Hwang HS et al. Involvement of the TNF-a Pathway in TKI Resistance and Suggestion of TNFR1 as a Predictive Biomarker for TKI Responsiveness in Clear Cell Renal Cell Carcinoma. J Korean Med Sci 35:e31 (2020). PubMed: 32030920
- Vial J et al. The Ectodysplasin receptor EDAR acts as a tumor suppressor in melanoma by conditionally inducing cell death. Cell Death Differ 26:443-454 (2019). PubMed: 29855541
- Vickman RE et al. Cholesterol Sulfotransferase SULT2B1b Modulates Sensitivity to Death Receptor Ligand TNFa in Castration-Resistant Prostate Cancer. Mol Cancer Res 17:1253-1263 (2019). PubMed: 30824526
