Overview

  • Product name
    Anti-TNF Receptor I antibody
    See all TNF Receptor I primary antibodies
  • Description
    Rabbit polyclonal to TNF Receptor I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.

  • Positive control
    • HeLa heat shocked cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
IHC-P 1/1000. Antigen retrieval is not essential but may optimise staining.

Incubate for 1 hour at RT.

Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 17210691

Target

  • Function
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease
    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications
    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

Images

  • Western blot analysis of mouse RAW 264 cell lysate.
  • Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells

    Secondary
    An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Observed band size: 52 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa (possible non-specific binding), 48 kDa (possible non-specific binding)



    Blocking Step :5% Milk + 1% BSA for 16 hours at 4°C
    Gel Running Conditions : Reduced, Denaturing 12% Bis-Tris

    See Abreview

  • ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Zhang C  et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol Oncol N/A:N/A (2018). WB . Read more (PubMed: 29377600) »
  • Annibaldi A  et al. Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2. Mol Cell 69:566-580.e5 (2018). Read more (PubMed: 29452637) »
See all 59 Publications for this product

Customer reviews and Q&As

1-10 of 34 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

I am sorry that there seems to be a problem with the current lot of ab19139 that you have. If you can provide me with the Abcam order number for this vial of ab19139, I will be happy to send a replacement vial out to you.

Looking forward to your reply.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Intestinal epithelial cell)
Gel Running Conditions
Reduced Denaturing (6-15% gradient gel)
Loading amount
20 µg
Specification
Intestinal epithelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 01 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 19 2014

Question
Answer

Our guarantee (www.abcam.com/abpromise) will cover pig reactivity for the TNFR1 antibody ab19139, but we do not have the data that demonstrated cross-reactivity. A customer several years ago used this antibody to stain sections of pig placenta and reported positive results but we regrettably did not obtain the protocol or data.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW264.7)
Specification
RAW264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Apr 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Standard
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Dec 17 2012

Answer

Vielen Dank für Ihre Anfrage.
Die meisten unserer Antikörper wurden nur unter denaturierenden/ reduzierenden WB Bedingungen getestet. Ich möchte daher vorschlagen, die Probe 5 min zu kochen und ein reduzierendes Gel zu verwenden.
Unsere Standartprotokolle finden Sie auch auf unserer Webseite, ich habe es Ihnen aber auch an diese Email gehängt. Desweiteren möchte ich vorschlagen, sich die entsprechenden Publikationen auf unserem Datenblatt anzusehen.
Falls es dann trotzdem zu Problemen kommt (zB. keine Banden, unerklärbare Banden, falsche Höhe der Bande, Antikörper muss viel zu hoch eingesetzt werden, etc.), melden sie sich bitte bei uns, damit wir das Problem zusammen lösen können, bzw. Sie von Ihrer Garantie Gebrauch machen können.
Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.
Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Question
Answer

Vielen Dank für Ihren Anruf.

Ich haben den folgenden Artikel gefunden, welcher trotz Trypsinisierung ein gutes Signal bekommt (und das Signal sogar gegen eine nicht-trypsinisierte Probe vergleicht):

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094030/

Ich hoffe, dies ist Ihnen hilfreich. Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

Read More

Answer

Thank you for contacting us.

We currently only carry this one anti-TNFR FITC conjugated.

OurAnti-TNF Receptor I antibody ab19139 has been tested to react with human tissues in a number of applications. While this product is not conjugated we do offer our EasyLink conjugation kits.Our EasyLink antibody conjugation kits provide asimple and quick linking process. We are also running an August promotion for 25% off these kits by using this code: NEW-T7193.

Our entire list of EasyLink conjugation kits is available at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

Our 3x10ug EasyLink FITC kit may be found at this link:

https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10%C2%B5g-FITC-ab102885.html

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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1-10 of 34 Abreviews or Q&A

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