Anti-TNF Receptor I antibody (ab19139)
- Datasheet
- References (59)
- Protocols
Overview
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Product nameAnti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies -
DescriptionRabbit polyclonal to TNF Receptor I
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
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Immunogen
Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.
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Positive control
- HeLa heat shocked cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab19139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa. | |
| IHC-P | 1/1000. Antigen retrieval is not essential but may optimise staining. Incubate for 1 hour at RT. |
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| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 17210691 |
Target
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FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
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Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5 -
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats. -
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
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Database links
- Entrez Gene: 282527 Cow
- Entrez Gene: 7132 Human
- Entrez Gene: 21937 Mouse
- Entrez Gene: 397020 Pig
- Entrez Gene: 25625 Rat
- Omim: 191190 Human
- SwissProt: O19131 Cow
- SwissProt: P19438 Human
see all -
Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
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Western blot - Anti-TNF Receptor I antibody (ab19139)Western blot analysis of mouse RAW 264 cell lysate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-TNF Receptor I antibody (ab19139)This image is courtesy of an anonymous AbreviewAnti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells
Secondary
An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 48 kDa (possible non-specific binding)
Blocking Step :5% Milk + 1% BSA for 16 hours at 4°C
Gel Running Conditions : Reduced, Denaturing 12% Bis-Tris -
Flow Cytometry - Anti-TNF Receptor I antibody (ab19139)Image courtesy of an anonymous Abreview.ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Protocols
References
This product has been referenced in:
- Zhang C et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol Oncol N/A:N/A (2018). WB . Read more (PubMed: 29377600) »
- Annibaldi A et al. Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2. Mol Cell 69:566-580.e5 (2018). Read more (PubMed: 29452637) »
