Key features and details
- Rabbit polyclonal to TNF Receptor I
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies
DescriptionRabbit polyclonal to TNF Receptor I
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- WB: Mouse liver lysate. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) and HaCaT (human keratinocyte cell line) cells. IHC: Mouse back skin.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 50.5 kDa.|
FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
- CD120a antibody
- FPF antibody
- MGC19588 antibody
Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution + mouse liver lysate at 15 µg
Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 50.5 kDa
Blocking: 5% skimmed milk in 1X TBST.
Primary incubation: 2 hours at room temperature
2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (red) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is APC Goat Anti-Rabbit used at 1/200 dilution. The nuclear counter stain is DAPI (blue)(panel A). The merged images are shown in panel C.
2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is FITC Goat Anti-Rabbit used at 1/200 diliution. The nuclear counter stain is DAPI (blue)(panel A) used at 1/40,000 dilution. The merged images are shown in panel C.
Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF. The secondary antibody is FITC Goat Anti-Rabbit at a 1/50 dilution.
Sections of mouse back skin were stained for TNF Receptor I with ab223352 at a dilution of 1/100 in immunohistochemical analysis.
The secondary antibody was FITC Goat Anti-Rabbit (green) at a 1/50 dilution.
ab223352 has not yet been referenced specifically in any publications.