Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1651AY] to TNF Receptor I - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-TNF Receptor I antibody [EP1651AY] - BSA and Azide free
See all TNF Receptor I primary antibodies
DescriptionRabbit monoclonal [EP1651AY] to TNF Receptor I - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human TNF Receptor I aa 1-100 (extracellular). The exact sequence is proprietary.
ab247368 is the carrier-free version of ab68160 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247368 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab247368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
- CD120a antibody
- FPF antibody
- MGC19588 antibody
Anti-TNF Receptor I antibody [EP1651AY] (ab68160) at 1/500 dilution + human brain tissue lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
This data was developed using ab68160, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247368 has not yet been referenced specifically in any publications.