Recombinant Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)
![Western blot - Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)](https://www.abcam.com/ps/products/259/ab259817/Images/ab259817-469942-anti-tnf-receptor-i-antibody-epr2374265-western-blot-cervix.jpg "Western blot - Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23742-65] to TNF Receptor I
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TNF Receptor I antibody [EPR23742-65]
See all TNF Receptor I primary antibodies -
Description
Rabbit monoclonal [EPR23742-65] to TNF Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HeLa, A549, HeLa and HepG2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23742-65 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 50 kDa.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 50 kDa. |
Target
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
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Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
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Western blot - Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)All lanes : Anti-TNF Receptor I antibody [EPR23742-65] (ab259817) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : TNFRSF1A (TNF Receptor I) knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lu carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST
Lanes 1-3: Merged signal (red and green). Green - ab259817 observed at 51 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
ab259817 Anti-TNF Receptor I antibody [EPR23742-65] was shown to specifically react with TNF Receptor I in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265972 (knockout cell lysate ab257751) was used. Wild-type and TNF Receptor I knockout samples were subjected to SDS-PAGE. ab259817 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)](https://www.abcam.com/ps/products/259/ab259817/Images/ab259817-469943-anti-tnf-receptor-i-antibody-epr2374265-western-blot-adenocarcinoma.jpg)
Western blot - Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)All lanes : Anti-TNF Receptor I antibody [EPR23742-65] (ab259817) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 3 : Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The difference in target band size observed in HeLa and HepG2 cell lysates is caused by glycosylation.
Negative control: Raji (PMID: 1966549; PMID: 2158104).
Exposure time: 81 seconds
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![Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)](https://www.abcam.com/ps/products/259/ab259817/Images/ab259817-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-TNF Receptor I antibody [EPR23742-65] (ab259817)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab259817 has not yet been referenced specifically in any publications.