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  1. Link

    tnf-receptor-i-antibody-h398-ab194814.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-TNF Receptor I antibody [H398] (ab194814)

  • Datasheet
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Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)
  • Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)

Key features and details

  • Mouse monoclonal [H398] to TNF Receptor I
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-TNF Receptor I antibody [H398]
    See all TNF Receptor I primary antibodies
  • Description

    Mouse monoclonal [H398] to TNF Receptor I
  • Host species

    Mouse
  • Specificity

    ab194814 recognizes the extracellular domain of TNF Receptor I. The antibody blocks biological activity of both natural and recombinant Human TNF alpha and TNF beta.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TNF Receptor I.
    Database link: P19438

  • Positive control

    • Flow Cyt: Human blood.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.095% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab194814 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is> 95% (by SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    H398
  • Isotype

    IgG2a
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Necrosis Factors
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • TNF ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Cancer
    • Cell Death
    • Necroptosis

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human TNF Receptor I protein (Active) (ab224647)
  • Related Products

    • Recombinant human TNF Receptor I protein (ab157278)
    • Recombinant human TNF Receptor I protein (Fc Chimera Active) (ab174046)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 1 - 4 µg/ml.
Notes
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

Target

  • Function

    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease

    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain

    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications

    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Target information above from: UniProt accession P19438 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7132 Human
    • Omim: 191190 Human
    • SwissProt: P19438 Human
    • Unigene: 279594 Human
    • Unigene: 713833 Human
    • Alternative names

      • CD120a antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55-R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    Images

    • Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)
      Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)

      Flow cytometry surface staining pattern of human peripheral whole blood stained using ab194814 purified antibody at a concentration in sample of 3 µg/mL GAM APC.

    • Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)
      Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)

      Separation of human monocytes (red-filled) from CD120a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab194814 purified antibody at concentration in sample of 3 µg/mL GAM APC.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab194814? Please let us know so that we can cite the reference in this datasheet.

    ab194814 has been referenced in 1 publication.

    • Beliu G  et al. Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy. Commun Biol 2:261 (2019). PubMed: 31341960

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