Anti-TNF Receptor I antibody [H398] (ab194814)
![Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)](https://www.abcam.com/ps/products/194/ab194814/Images/ab194814-453138-anti-tnf-receptor-i-antibody-h398-flow-cytometry-scatter-plot-blood-human.jpg "Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)")
Key features and details
- Mouse monoclonal [H398] to TNF Receptor I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
-
Product name
Anti-TNF Receptor I antibody [H398]
See all TNF Receptor I primary antibodies -
Description
Mouse monoclonal [H398] to TNF Receptor I -
Host species
Mouse -
Specificity
ab194814 recognizes the extracellular domain of TNF Receptor I. The antibody blocks biological activity of both natural and recombinant Human TNF alpha and TNF beta. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human TNF Receptor I.
Database link: P19438 -
Positive control
- Flow Cyt: Human blood.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.095% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab194814 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is> 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
H398 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
Target
-
Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
-
Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
-
Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
-
Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)
Flow cytometry surface staining pattern of human peripheral whole blood stained using ab194814 purified antibody at a concentration in sample of 3 µg/mL GAM APC.
-
![Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)](https://www.abcam.com/ps/products/194/ab194814/Images/ab194814-453137-anti-tnf-receptor-i-antibody-h398-flow-cytometry-histogram-blood-human.jpg)
Flow Cytometry - Anti-TNF Receptor I antibody [H398] (ab194814)Separation of human monocytes (red-filled) from CD120a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab194814 purified antibody at concentration in sample of 3 µg/mL GAM APC.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab194814 has been referenced in 1 publication.
- Beliu G et al. Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy. Commun Biol 2:261 (2019). PubMed: 31341960
