Anti-TNF Receptor I antibody [H398] (ab194814)
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Overview
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Product name
Anti-TNF Receptor I antibody [H398]
See all TNF Receptor I primary antibodies -
Description
Mouse monoclonal [H398] to TNF Receptor I -
Host species
Mouse -
Specificity
ab194814 recognizes the extracellular domain of TNF Receptor I. The antibody blocks biological activity of both natural and recombinant Human TNF alpha and TNF beta. -
Tested applications
Suitable for: Functional Studies, Blocking, IHC-P, IHC-Fr, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human TNF Receptor I.
Database link: P19438
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.095% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab194814 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is> 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
H398 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab194814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. | |
| Blocking | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
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Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
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Datasheets and documents
References
ab194814 has not yet been referenced specifically in any publications.
