Key features and details
- Mouse monoclonal [H398] to TNF Receptor I
- Suitable for: Functional Studies, Blocking, IHC-P, IHC-Fr, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-TNF Receptor I antibody [H398]
See all TNF Receptor I primary antibodies
DescriptionMouse monoclonal [H398] to TNF Receptor I
Specificityab194814 recognizes the extracellular domain of TNF Receptor I. The antibody blocks biological activity of both natural and recombinant Human TNF alpha and TNF beta.
Tested applicationsSuitable for: Functional Studies, Blocking, IHC-P, IHC-Fr, IP, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human TNF Receptor I.
Database link: P19438
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.095% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab194814 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is> 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab194814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
- CD120a antibody
- FPF antibody
- MGC19588 antibody
ab194814 has been referenced in 1 publication.
- Beliu G et al. Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy. Commun Biol 2:261 (2019). PubMed: 31341960