• Product name
    Anti-TNF Receptor II antibody
    See all TNF Receptor II primary antibodies
  • Description
    Rabbit polyclonal to TNF Receptor II
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse TNF Receptor II aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P25119

  • Positive control
    • Thymus



Our Abpromise guarantee covers the use of ab15563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 2 µg/ml.
IHC-P 1/50.


  • Function
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similarities
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor binding protein 2 antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all


  • ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections).
  • Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg

    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa

    Exposure time: 2 minutes

    Blocked with 5% milk for 1 hour at 22°C.

    See Abreview


This product has been referenced in:
  • Aghagolzadeh P  et al. Calcification of vascular smooth muscle cells is induced by secondary calciprotein particles and enhanced by tumor necrosis factor-a. Atherosclerosis 251:404-414 (2016). Read more (PubMed: 27289275) »
  • Weinberg MS  et al. Opposing actions of hippocampus TNFa receptors on limbic seizure susceptibility. Exp Neurol 247:429-37 (2013). Read more (PubMed: 23333565) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A


Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab121520, the datasheet states "Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol."

For the other 2 antibodies I have contacted the lab to find out what is recommended. I will let you know.

Could you please describe to me which problem you had with each antibody as well as protocol information and what tissue samples were used, that would be of great help to look into this in greater detail.

I look forward to hear back from you and assist you further.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (CD14+)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Flow Cytometry
Human Cell (Monocytes from Apheresis)
Monocytes from Apheresis
BD Cytofix/Cytoperm 20 min
Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 23 2011


Thank you for your inquiry. Unfortunately, we are not able to provide the blocking peptides for these antibodies. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More


Thank you for contacting us. This antibody has been successfully tested in Dog and Human species in our lab, but has not been tested in Mouse. However, due to the high sequence homology between the protein’s immunogens, it is predicted to react in Mouse too. As we do not have any data to support its reactivity, we are not able to assure it will work with mouse, but it is very likely to react. However, if you wish to test ab15563 in Mouse, we can offer you an Abreview discount to the value of 1 free primary antibody. Therefore, if you buy now ab15563, you test it in Mouse species, and submit feedback to us in the form of an Abreview, I will be happy to offer a discount off a future purchase to the value of one free primary antibody. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab15563 in Mouse species. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering. 2. Purchase the products either by phone, fax, or online (www.abcam.com). 3. Test it in Mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. In case you are not interested in testing this antibody in Mouse, there are, two other antibodies form our catalogue that could be of interest to you. Both recognise TNF-Receptor II, and are previously tested in Mouse. I recommend you to have a look at their datasheet and see if they fit your experiment requirements: https://www.abcam.com/TNF-Receptor-II-antibody-HM102-ab7369.html https://www.abcam.com/TNF-Receptor-II-antibody-ab7364.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry. I was not able to obtain a range for the sequence of this immunogen, but by looking closely at this antibody's data sheet in immunogen section, it states that the immunogen is a: "Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal)." I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (TF-1 cells)
Total protein in input
30 µg
TF-1 cells
Immuno-precipitation step
Protein A/G

Dr. Alison Taylor

Verified customer

Submitted Nov 08 2006


Thank you for your enquiry. The following antibodies fir your specifications (although we don't know if they have been tested on brain samples), please take a look at the online datasheets for additional information. Please contact us again if you have questions regarding these products. TNF Receptor I: ab7630, ab7743, ab19139 TNF Receptor II: ab7744, ab15563, ab19301

Read More


Thank you for your email and I'm sorry to hear that you are experiencing difficulty with these two antibodies. For ab15563, it is suggested on the online datasheet to use thymus as a positive control. Also, TNF receptors are present on nearly all cell types with few exceptions such as erythrocytes and resting T-cells. The type I (p55) receptor is found on most cell types whereas the type II (p75) receptor seems more restricted to haematopoietic cells. So for TNF receptor I (p55) there are a lot of options to use as positive control in Western blotting experiments, for example KYM-1 cells can be used. This cell-line should also be a good option for TNF receptor II (p75). Since you are not seeing a signal with ab7743, I would suggest increasing the concentrations of both the primary and secondary antibodies and also increase the incubation periods; make sure you load at least 20-30 ug of lysate; and ensure the protein transferred properly to the membrane. To help decrease the number of non-specific bands that you are seeing with ab15563, decrease the concentration of the primary. Often there are multiple bands if the concentration is too high. Also, make sure to run a secondary only control (no primary) to ensure that these bands are not due to the secondary antibody. I hope that this is helpful to you and if you have any more questions, please do contact us again.

Read More


Sign up