Product nameAnti-TNF Receptor II antibody
See all TNF Receptor II primary antibodies
DescriptionRabbit polyclonal to TNF Receptor II
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
|IP||Use a concentration of 2 µg/ml.|
FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Sequence similaritiesContains 4 TNFR-Cys repeats.
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections).
Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 2 minutes
Blocked with 5% milk for 1 hour at 22°C.
This product has been referenced in:
- Aghagolzadeh P et al. Calcification of vascular smooth muscle cells is induced by secondary calciprotein particles and enhanced by tumor necrosis factor-a. Atherosclerosis 251:404-414 (2016). Read more (PubMed: 27289275) »
- Weinberg MS et al. Opposing actions of hippocampus TNFa receptors on limbic seizure susceptibility. Exp Neurol 247:429-37 (2013). Read more (PubMed: 23333565) »