Key features and details
- Rabbit polyclonal to TNF Receptor II - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TNF Receptor II antibody - C-terminal
See all TNF Receptor II primary antibodies
DescriptionRabbit polyclonal to TNF Receptor II - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human TNF Receptor II (C terminal).
Database link: P20333
- RAW264.7, HT-29 and LOVO cell extracts; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Sequence similaritiesContains 4 TNFR-Cys repeats.
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
All lanes : Anti-TNF Receptor II antibody - C-terminal (ab196941) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : HT-29 cell extract
Lane 3 : LOVO cell extract
Predicted band size: 48 kDa
Immunofluorescent analysis of HeLa cells labeling TNF Receptor II with ab196941 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
ab196941 has not yet been referenced specifically in any publications.