Recombinant Anti-TNF Receptor II antibody [EPR1653] (ab109322)
![Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-449573-anti-tnf-receptor-ii-antibody-epr1653-westernblot-human-mouse-rat.jpg "Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1653] to TNF Receptor II
- Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TNF Receptor II antibody [EPR1653]
See all TNF Receptor II primary antibodies -
Description
Rabbit monoclonal [EPR1653] to TNF Receptor II -
Host species
Rabbit -
Specificity
We do not guarantee IHC-P for mouse and rat species. -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Jurkat cells; ICC: Jurkat cells; IHC-P: Human breast carcinoma tissue; WB: Jurkat, NIH/3T3 and C6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1653 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-TNF Receptor II antibody [EPR1653] (ab199821)
- Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)
- HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)
- PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
- PerCP Anti-TNF Receptor II antibody [EPR1653] (ab220515)
- Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
- Anti-TNF Receptor II antibody [EPR1653] - BSA and Azide free (ab226058)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/50.
For best results in ICC/IF please use 100% Methanol fixation. |
| WB |
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).
|
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| IP |
1/10 - 1/100.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. We do not guarantee IHC-P for mouse and rat species. |
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/50. For best results in ICC/IF please use 100% Methanol fixation. |
|
WB
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa). |
|
IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. We do not guarantee IHC-P for mouse and rat species. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7133 Human
- Entrez Gene: 21938 Mouse
- Entrez Gene: 156767 Rat
- Omim: 191191 Human
- SwissProt: P20333 Human
- SwissProt: P25119 Mouse
- SwissProt: Q80WY6 Rat
- Unigene: 256278 Human
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Alternative names
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
see all
Images
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Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)All lanes : Anti-TNF Receptor II antibody [EPR1653] (ab109322) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDaThe molecular mass observed is consistent with what has been described in the literatures (PMID: 8069916, 25609706).
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![Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-9-anti-tnf-receptor-II-antibody-epr1653-flowcytometry-jurkat-human.jpg)
Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-8-anti-tnf-receptor-II-antibody-epr1653-immunocytochemistry-jurkat-human.jpg)
Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/50 dilution (2.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-7-anti-tnf-receptor-ii-antibody-epr1653-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TNF Receptor II with Purified ab109322 at 1/150 dilution (0.89 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-10-anti-tnf-receptor-II-antibody-epr1653-immunoprecipitation-jurkat-human.jpg)
Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)Purified ab109322 at 1/20 dilution (0.6µg) immunoprecipitating TNF Receptor II in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab109322 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109322 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 73 kDa -
![Anti-TNF Receptor II antibody [EPR1653] (ab109322)](https://www.abcam.com/ps/products/109/ab109322/Images/ab109322-6-benefits-of-recombinant-antibodies.png)
Anti-TNF Receptor II antibody [EPR1653] (ab109322)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab109322 has been referenced in 15 publications.
- Xu M et al. Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation. Mol Metab 34:112-123 (2020). PubMed: 32180551
- Wang F et al. Diet-Induced Obesity Mice Execute Pulmonary Cell Apoptosis via Death Receptor and ER-Stress Pathways after E. coli Infection. Oxid Med Cell Longev 2020:6829271 (2020). PubMed: 32685099
- Bradley JR et al. Tumor necrosis factor receptor-2 signaling pathways promote survival of cancer stem-like CD133+ cells in clear cell renal carcinoma. FASEB Bioadv 2:126-144 (2020). PubMed: 32123862
- Hu X et al. Tumor necrosis factor-alpha aggravates gliosis and inflammation of activated retinal Müller cells. Biochem Biophys Res Commun 531:383-389 (2020). PubMed: 32800547
- Wang X et al. Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis. Mediators Inflamm 2019:2945083 (2019). PubMed: 31885495