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Thanks for the WB you sent us. It really is incredibly impressive and clear. I’ll appreciate it if you send us extract from one or more of these cell lines so that we can use it as control as we get nothing like the signals shown in your picture
Please let me know whether any of the cell lines you used is transfected with NTFRII (thus overexpressing it). Also, let us know how many micrograms of extract you loaded on each lane of the blot you sent us.
Asked on Oct 18 2012
Thank you very much for your reply.
I've asked the lab whether the cells are transfected, and how many micrograms of lysate were loaded.
I'd be happy to send a positive control lysate, however I can't send all of the lysates shown in the image. We have the RAW 264.7 lysate ab7187 or NIH/3T3 lysate ab7179-
Which of these would you prefer? I'll be happy to send this out promptly. Please forward me your original PO or order number and I will ship it to the same address.
I look forward to hearing from you, and I will send you the information from the lab once I receive it. Please let me know if you have further questions.
Answered on Oct 18 2012