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Thanks for the WB you sent us. It really is incredibly impressive and clear. I’ll appreciate it if you send us extract from one or more of these cell lines so that we can use it as control as we get nothing like the signals shown in your picture
Please let me know whether any of the cell lines you used is transfected with NTFRII (thus overexpressing it). Also, let us know how many micrograms of extract you loaded on each lane of the blot you sent us.
Asked on Oct 18 2012
Thank you again for your email, and for your patience.
I've heard back from the lab, and they load 10 ug of protein per lane. None of the cell lines are transfected, and they use the general Epitomics Western blot protocol-
Please let me know which positive control lysate you would like to try. Have a nice day.
Answered on Oct 18 2012