Product nameAnti-TNF Receptor II antibody [EPR1653] (PerCP)
See all TNF Receptor II primary antibodies
DescriptionRabbit monoclonal [EPR1653] to TNF Receptor II (PerCP)
ConjugationPerCP. Ex: 482nm, Em: 675nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Flow Cyt: Jurkat cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-TNF Receptor II antibody [EPR1653] (ab109322)
- Anti-TNF Receptor II antibody [EPR1653] (Alexa Fluor® 488) (ab199821)
- Anti-TNF Receptor II antibody [EPR1653] (Alexa Fluor® 647) (ab199822)
- Anti-TNF Receptor II antibody [EPR1653] (HRP) (ab200006)
- Anti-TNF Receptor II antibody [EPR1653] (Phycoerythrin) (ab209582)
- Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab220515 in the following tested applications.
The cellular localisation of this product has been verified in ICC/IF.
ab222107 - Rabbit monoclonal IgG (PerCP), is suitable for use as an isotype control with this antibody.
FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Sequence similaritiesContains 4 TNFR-Cys repeats.
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
Overlay histogram showing Jurkat cells stained with ab220515 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220515, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
ab220515 has not yet been referenced specifically in any publications.