Product nameAnti-TNFAIP3 antibody [59A426]
See all TNFAIP3 primary antibodies
DescriptionMouse monoclonal [59A426] to TNFAIP3
Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fusion protein of full length TNFAIP3 (Human).
EpitopeThe epitope has been mapped to the C-terminal portion of A20, amino acids 440-790.
- Whole cell lysate from Daudi cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab13597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 19637364|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 70 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use a concentration of 1 - 2 µg/ml.|
FunctionUbiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similaritiesBelongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
DomainThe A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- A20 antibody
- AISBL antibody
- MGC104522 antibody
Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue labelling TNFAIP3 with ab13597 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This product has been referenced in:
- Meng Z et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol 198:820-831 (2017). Read more (PubMed: 27986908) »
- Xu H et al. Elevated serum A20 is associated with severity of chronic hepatitis B and A20 inhibits NF-?B-mediated inflammatory response. Oncotarget 8:38914-38926 (2017). Read more (PubMed: 28473659) »