Overview

  • Product name

  • Description

    Rabbit polyclonal to TNFAIP3
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total TNFAIP3 protein.
  • Tested applications

    Suitable for: IHC-Fr, ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A synthesized peptide derived from the internal region of human TNFAIP3.

  • Positive control

    • HepG2 cells and extracts from RAW264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ELISA
WB
ICC/IF
  • Application notes
    ELISA: 1/20000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 90 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
    • Sequence similarities

      Belongs to the peptidase C64 family.
      Contains 7 A20-type zinc fingers.
      Contains 1 OTU domain.
    • Domain

      The A20-type zinc fingers mediate the ubiquitin ligase activity.
      The OTU domain mediates the deubiquitinase activity.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • A20 antibody
      • AISBL antibody
      • MGC104522 antibody
      • MGC138687 antibody
      • MGC138688 antibody
      • OTU domain containing protein 7C antibody
      • OTU domain-containing protein 7C antibody
      • OTUD7C antibody
      • Putative DNA binding protein A20 antibody
      • Putative DNA-binding protein A20 antibody
      • TNAP3_HUMAN antibody
      • TNF alpha-induced protein 3 antibody
      • TNFA1P2 antibody
      • TNFAIP 3 antibody
      • TNFAIP3 (A20) antibody
      • TNFAIP3 antibody
      • Tumor necrosis factor alpha induced protein 3 antibody
      • Tumor necrosis factor alpha-induced protein 3 antibody
      • Tumor necrosis factor induced protein 3 antibody
      • Tumor necrosis factor inducible protein A20 antibody
      • tumor necrosis factor, alpha-induced protein 3 antibody
      • Zinc finger protein A20 antibody
      see all

    Images

    • All lanes : Anti-TNFAIP3 antibody (ab74037) at 1/500 dilution

      Lane 1 : Extracts from RAW264.7 cells
      Lane 2 : Extracts from RAW264.7 cells with immunizing peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

    • Immunofluorescence analysis of TNFAIP3 in HepG2 cells using ab74037 at a 1/500 dilution.
      Left image: untreated.
      Right image: treated with immunizing peptide.
    • ab74037, at 1/50, staining TNFAIP3 in mouse tissue sections by immunohistochemistry (frozen sections).

      See Abreview

    References

    This product has been referenced in:

    • Li M  et al. Daphnetin inhibits inflammation in the NZB/W F1 systemic lupus erythematosus murine model via inhibition of NF-?B activity. Exp Ther Med 13:455-460 (2017). WB ; Mouse . Read more (PubMed: 28352315) »
    • Wu J  et al. CCCTC-binding factor inhibits breast cancer cell proliferation and metastasis via inactivation of the nuclear factor-kappaB pathway. Oncotarget 8:93516-93529 (2017). Read more (PubMed: 29212169) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Question
    Answer

    ab74037 to be tested in IHC-P: discount code ****
    ab109018 to be tested in ICC/IF: discount code *****

    Expiration date for both codes: DD MM YYYY

    I am very pleased to hear you would like to accept our offer and testab74037 in IHC-P and ab109018in ICC/IF.Each code will give you1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab74037 in IHC-P and ab109018 in ICC/IF and include the corresponding code in the “Additional Comments” section so we know the Abreview is for this promotion.

    For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting us.

    Could you please precise in wihich application and species you like to test these 2 antibodies?

    A testing discount can be issued only for untested application or species.

    Thank you.

    Read More

    Answer

    Thank you for contacting us.

    Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of any of the antibodies you are interested in.

    However, ab74037 has been validated with mouse samples by IHC (in frozen sections), and therefore it is guaranteed to work in this application, so if it did not work following the datasheet indications, I would be happy to send a replacement or reimbursement as stated by our Abpromise guarantee.

    Unfortunately ab92324 has been proved not to react with mouse samples, so I would not recommend using this antibody for your purpose. On the other hand ab111192 has only been validated to work with human samples in IHC (paraffin embedded sections).

    My recommendation would be using ab74037 with mouse tissue if you are using IHC-Fr. If you wish to use it for IHC-P I would be happy to offer a discount code to test it in this technique and submit your feedback to us.

    Therefore, I can offer a discount off a future purchase if you buy ab74037 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please reply to me letting me know before purchasing the antibody.

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab45366. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab92324. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Thymus)
    Specification
    Thymus
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C

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    Verified customer

    Submitted Aug 17 2009

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