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  1. Link

    tnfaip3-antibody-epr2663-ab92324.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TNFAIP3 antibody [EPR2663] (ab92324)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (24)

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Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
  • Anti-TNFAIP3 antibody [EPR2663] (ab92324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2663] to TNFAIP3
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE PerCP

You may also be interested in

Protein
Product image
Recombinant Human TNFAIP3 protein (ab132088)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human TNFAIP3 knockout HeLa cell line (ab265983)

View more associated products

Overview

  • Product name

    Anti-TNFAIP3 antibody [EPR2663]
    See all TNFAIP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR2663] to TNFAIP3
  • Host species

    Rabbit
  • Specificity

    Mouse species is recommended based on WB results, we do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: WEHI-3 treated with TNF (ab9642), Jurkat treated with TNF (ab9642) + TPA, Jurkat treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, HeLa, A549 and Daudi cell lysates. IHC-P: Human kidney tissue. ICC/IF: Daudi and HeLa cells. Flow Cyt (intra): HepG2 and Daudi cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2663
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-TNFAIP3 antibody [EPR2663] (ab197068)
    • Alexa Fluor® 488 Anti-TNFAIP3 antibody [EPR2663] (ab197541)
    • PerCP Anti-TNFAIP3 antibody [EPR2663] (ab220511)
    • Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human TNFAIP3 knockout HeLa cell line (ab265983)
    • Human TNFAIP3 knockout A549 cell line (ab266945)
    • Human TNFAIP3 knockout A549 cell line (ab266946)
  • KO cell lysates

    • Human TNFAIP3 knockout HeLa cell lysate (ab257112)
    • Human TNFAIP3 knockout A549 cell lysate (ab257113)
    • Human TNFAIP3 knockout A549 cell lysate (ab257114)
  • KO cell pellets

    • Human TNFAIP3 knockout HeLa cell pellet (ab278935)
    • Human TNFAIP3 knockout A549 cell pellet (ab278936)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)
  • Recombinant Protein

    • Recombinant Human TNFAIP3 protein (ab132088)
  • Related Products

    • Recombinant human TNF alpha protein (ab9642)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50 - 1/500.
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
IHC-P (1)
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Mouse species is recommended based on WB results, we do not guarantee IHC-P for mouse.

Notes
ICC/IF
1/50 - 1/500.
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Mouse species is recommended based on WB results, we do not guarantee IHC-P for mouse.

Target

  • Function

    Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
  • Sequence similarities

    Belongs to the peptidase C64 family.
    Contains 7 A20-type zinc fingers.
    Contains 1 OTU domain.
  • Domain

    The A20-type zinc fingers mediate the ubiquitin ligase activity.
    The OTU domain mediates the deubiquitinase activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P21580 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7128 Human
    • Entrez Gene: 21929 Mouse
    • Omim: 191163 Human
    • SwissProt: P21580 Human
    • SwissProt: Q60769 Mouse
    • Unigene: 211600 Human
    • Unigene: 116683 Mouse
    • Alternative names

      • A20 antibody
      • AISBL antibody
      • MGC104522 antibody
      • MGC138687 antibody
      • MGC138688 antibody
      • OTU domain containing protein 7C antibody
      • OTU domain-containing protein 7C antibody
      • OTUD7C antibody
      • Putative DNA binding protein A20 antibody
      • Putative DNA-binding protein A20 antibody
      • TNAP3_HUMAN antibody
      • TNF alpha-induced protein 3 antibody
      • TNFA1P2 antibody
      • TNFAIP 3 antibody
      • TNFAIP3 (A20) antibody
      • TNFAIP3 antibody
      • Tumor necrosis factor alpha induced protein 3 antibody
      • Tumor necrosis factor alpha-induced protein 3 antibody
      • Tumor necrosis factor induced protein 3 antibody
      • Tumor necrosis factor inducible protein A20 antibody
      • tumor necrosis factor, alpha-induced protein 3 antibody
      • Zinc finger protein A20 antibody
      see all

    Images

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      All lanes : Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : TNFAIP3 knockout A549 cell lysate
      Lane 3 : Wild-type HeLa cell lysate
      Lane 4 : TNFAIP3 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa



      Lanes 1- 4: Merged signal (red and green). Green - ab92324 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab92324 was shown to react with TNFAIP3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab266946 (knockout cell lysate ab257114) was used. Wild-type A549 and TNFAIP3 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with unpurified ab92324 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling TNFAIP with purified ab92324 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      All lanes : Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : TNFAIP3 knockout HeLa cell lysate
      Lane 3 : Jurkat cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate
      Lane 4 : Untreated Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 90 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control, ab8245 observed at 37 kDa.

      ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265983 (knockout cell lysate ab257112) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      All lanes : Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

      Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 2 : TNFAIP3 knockout A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate
      Lane 4 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 90 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266945 (knockout cell lysate ab257113) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      All lanes : Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/5000 dilution

      Lane 1 : WEHI-3 (Mouse leukemia lymphoblast) whole cell lysate
      Lane 2 : WEHI-3 treated with 20 ng/ml TNF alpha (ab9642) for 6 h

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 90 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/2000 dilution (unpurified) + Jurkat cell lysate - treated with TNF (ab9642) and TPA at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 90 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/3000 dilution (purified) + Jurkat cell lysate - treated with TNF (ab9642) and TPA at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 90 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with purified ab92324 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Immunocytochemsitry/Immunofluorescence analysis of Daudi cells labelling TNFAIP3 (red) with unpurified ab92324 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Immunocytochemsitry/Immunofluorescence analysis of Daudi cells labelling TNFAIP3 (red) with purified ab92324 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Overlay histogram showing HepG2 cells stained with unpurified ab92324 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92324, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Intracellular Flow Cytometry analysis of Daudi cells labelling TNFAIP3 with unpurified ab92324 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG. 

       

       

    • Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

      Intracellular Flow Cytometry analysis of Daudi cells labelling TNFAIP3 with purified ab92324 at 1/30 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG. 

       

       

    • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      All lanes : Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat cells treated with TNF (ab9642) and TPA
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 90 kDa

    • Anti-TNFAIP3 antibody [EPR2663] (ab92324)
      Anti-TNFAIP3 antibody [EPR2663] (ab92324)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (24)

    Publishing research using ab92324? Please let us know so that we can cite the reference in this datasheet.

    ab92324 has been referenced in 24 publications.

    • Zhang L  et al. Potential of the tumor-derived extracellular vesicles carrying the miR-125b-5p target TNFAIP3 in reducing the sensitivity of diffuse large B cell lymphoma to rituximab. Int J Oncol 58:N/A (2021). PubMed: 33887878
    • Su Y  et al. Mesenchymal stem cell-originated exosomal lncRNA HAND2-AS1 impairs rheumatoid arthritis fibroblast-like synoviocyte activation through miR-143-3p/TNFAIP3/NF-?B pathway. J Orthop Surg Res 16:116 (2021). PubMed: 33549125
    • Fu YW & Xu HZ Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts. BMC Oral Health 21:290 (2021). PubMed: 34092220
    • Chen J  et al. TNFAIP3 ameliorates the degeneration of inflammatory human nucleus pulposus cells by inhibiting mTOR signaling and promoting autophagy. Aging (Albany NY) 12:24242-24254 (2020). PubMed: 33226960
    • Yan K  et al. A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxia. Cell Prolif 53:e12778 (2020). PubMed: 32027437
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNFAIP3 antibody [EPR2663]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Breast tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

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    Submitted Dec 31 2019

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