Recombinant
RabMAb

Recombinant Anti-TNFAIP3 antibody [EPR2663] (Alexa Fluor® 647) (ab197068)

Overview

  • Product name

    Anti-TNFAIP3 antibody [EPR2663] (Alexa Fluor® 647)
    See all TNFAIP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR2663] to TNFAIP3 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TNFAIP3 aa 500-600. The exact sequence is proprietary.
    (Peptide available as ab175807)

  • Positive control

    • ICC/IF: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

Target

  • Function

    Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
  • Sequence similarities

    Belongs to the peptidase C64 family.
    Contains 7 A20-type zinc fingers.
    Contains 1 OTU domain.
  • Domain

    The A20-type zinc fingers mediate the ubiquitin ligase activity.
    The OTU domain mediates the deubiquitinase activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A20 antibody
    • AISBL antibody
    • MGC104522 antibody
    • MGC138687 antibody
    • MGC138688 antibody
    • OTU domain containing protein 7C antibody
    • OTU domain-containing protein 7C antibody
    • OTUD7C antibody
    • Putative DNA binding protein A20 antibody
    • Putative DNA-binding protein A20 antibody
    • TNAP3_HUMAN antibody
    • TNF alpha-induced protein 3 antibody
    • TNFA1P2 antibody
    • TNFAIP 3 antibody
    • TNFAIP3 (A20) antibody
    • TNFAIP3 antibody
    • Tumor necrosis factor alpha induced protein 3 antibody
    • Tumor necrosis factor alpha-induced protein 3 antibody
    • Tumor necrosis factor induced protein 3 antibody
    • Tumor necrosis factor inducible protein A20 antibody
    • tumor necrosis factor, alpha-induced protein 3 antibody
    • Zinc finger protein A20 antibody
    see all

Images

  • ab197068 staining TNFAIP3 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197068 at 1/50 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab197068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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