Anti-TNFAIP8 antibody (ab166804)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-TNFAIP8 antibody
See all TNFAIP8 primary antibodies -
DescriptionRabbit polyclonal to TNFAIP8
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide derived from internal sequence of Human TNFAIP8.
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Positive control
- HuvEc cell extract; Human breast tissue; A549 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 23 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/100 - 1/500. |
Target
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FunctionActs as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.
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Tissue specificityExpressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle.
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Sequence similaritiesBelongs to the TNFAIP8 family.
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Developmental stageExpressed at high levels in the fetal liver, lung and kidney.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 25816 Human
- Entrez Gene: 106869 Mouse
- GenBank: NP_055165.1 Human
- Omim: 612111 Human
- SwissProt: O95379 Human
- SwissProt: Q921Z5 Mouse
- Unigene: 656274 Human
- Unigene: 27740 Mouse
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Alternative names
- GG2 1 antibody
- Head and neck tumor and metastasis-related protein antibody
- MDC-3.13 antibody
see all
Images
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Western blot - Anti-TNFAIP8 antibody (ab166804)All lanes : Anti-TNFAIP8 antibody (ab166804) at 1/100 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunizing peptide
Predicted band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP8 antibody (ab166804)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling TNFAIP8 with ab166804 at 5 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP8 antibody (ab166804)Immunofluorescent analysis of A549 cells labeling TNFAIP8 with ab166804 at 1/100 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xing Y et al. TNFAIP8 promotes the proliferation and cisplatin chemoresistance of non-small cell lung cancer through MDM2/p53 pathway. Cell Commun Signal 16:43 (2018). Read more (PubMed: 30064446) »
- Hu R et al. Expression of tumor necrosis factor-a-induced protein 8 in stage III gastric cancer and the correlation with DcR3 and ERK1/2. Oncol Lett 11:1835-1840 (2016). Read more (PubMed: 26998086) »
