Recombinant Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10058(3)] to TNFAIP8
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TNFAIP8 antibody [EPR10058(3)]
See all TNFAIP8 primary antibodies -
Description
Rabbit monoclonal [EPR10058(3)] to TNFAIP8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Spleen (Human) Whole Cell Lysate - fetal normal tissue (ab29701) can be used as a positive control in WB. K562, A549 and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10058(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 21, 19 kDa (predicted molecular weight: 23 kDa).
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IP |
1/70.
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Notes |
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WB
1/1000. Detects a band of approximately 21, 19 kDa (predicted molecular weight: 23 kDa). |
IP
1/70. |
Target
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Function
Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. -
Tissue specificity
Expressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle. -
Sequence similarities
Belongs to the TNFAIP8 family. -
Developmental stage
Expressed at high levels in the fetal liver, lung and kidney. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 25816 Human
- GenBank: NP_055165.1 Human
- Omim: 612111 Human
- SwissProt: O95379 Human
- Unigene: 656274 Human
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Alternative names
- GG2 1 antibody
- Head and neck tumor and metastasis-related protein antibody
- MDC-3.13 antibody
see all
Images
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All lanes : Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 19,21 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.
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Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution + Human fetal spleen lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 19,21 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.
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TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract (Input) 10µg. Lane 2: ab195810 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab195810 has been referenced in 6 publications.
- Mandigo AC et al. Novel Oncogenic Transcription Factor Cooperation in RB-Deficient Cancer. Cancer Res 82:221-234 (2022). PubMed: 34625422
- Yang X et al. circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8. Int J Genomics 2021:3997045 (2021). PubMed: 34901263
- Zhong M et al. Expression of TIPE family members in human colorectal cancer. Oncol Lett 21:118 (2021). PubMed: 33376549
- Yang J et al. Long noncoding RNA DLEU2 drives the malignant behaviors of thyroid cancer through mediating the miR-205-5p/TNFAIP8 axis. Endocr Connect 10:471-483 (2021). PubMed: 33764889
- Zhong M et al. TNFAIP8 promotes the migration of clear cell renal cell carcinoma by regulating the EMT. J Cancer 11:3061-3071 (2020). PubMed: 32226521
- Wu S et al. TNFAIP8 promotes cisplatin resistance in cervical carcinoma cells by inhibiting cellular apoptosis. Oncol Lett 17:4667-4674 (2019). PubMed: 30944654