Recombinant Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10058(3)] to TNFAIP8 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free
See all TNFAIP8 primary antibodies -
Description
Rabbit monoclonal [EPR10058(3)] to TNFAIP8 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251212 is the carrier-free version of ab195810.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10058(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa (predicted molecular weight: 23 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa (predicted molecular weight: 23 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. -
Tissue specificity
Expressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle. -
Sequence similarities
Belongs to the TNFAIP8 family. -
Developmental stage
Expressed at high levels in the fetal liver, lung and kidney. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 25816 Human
- GenBank: NP_055165.1 Human
- Omim: 612111 Human
- SwissProt: O95379 Human
- Unigene: 656274 Human
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Alternative names
- GG2 1 antibody
- Head and neck tumor and metastasis-related protein antibody
- MDC-3.13 antibody
see all
Images
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All lanes : Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 19,21 kDa why is the actual band size different from the predicted?This data was developed using ab195810, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.
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Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution + Human fetal spleen lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 19,21 kDa why is the actual band size different from the predicted?This data was developed using ab195810, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.
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This data was developed using ab195810, the same antibody clone in a different buffer formulation.TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract (Input) 10µg. Lane 2: ab195810 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab251212 has been referenced in 1 publication.
- Chen Z et al. TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals. J Cell Mol Med 25:3361-3370 (2021). PubMed: 33682317