Key features and details
- Rabbit polyclonal to TNFRSF14/HVEM
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TNFRSF14/HVEM antibody
See all TNFRSF14/HVEM primary antibodies
DescriptionRabbit polyclonal to TNFRSF14/HVEM
Tested applicationsSuitable for: WB, ICC/IF, ICCmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TNFRSF14/HVEM (C terminal).
- Raji cell lysate. Raji cells.
This product was previously labelled as TNFRSF14
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|ICC||Use a concentration of 10 µg/ml.|
FunctionReceptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
Tissue specificityWidely expressed, with the highest expression in lung, spleen and thymus.
Sequence similaritiesContains 3 TNFR-Cys repeats.
- Information by UniProt
- HVEML antibody
- ATAR antibody
- CD270 antibody
All lanes : Anti-TNFRSF14/HVEM antibody (ab62462) at 2 µg/ml
Lane 1 : Raji cell lysate with no blocking peptide
Lane 2 : Raji cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Immunocytochemistry of TNFRSF14/HVEM in Raji cells with ab62462 antibody at 10 µg/ml.
Immunofluorescence of TNFRSF14/HVEM in Raji cells using ab62462 at 20 ug/ml.
ab62462 has been referenced in 1 publication.
- Duan L et al. HVEM/HIF-1a promoted proliferation and inhibited apoptosis of ovarian cancer cells under hypoxic microenvironment conditions. J Ovarian Res 13:40 (2020). PubMed: 32312328