Recombinant
RabMAb

Recombinant Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)

Overview

  • Product name

    Anti-TNFRSF14/HVEM antibody [EPR22122]
    See all TNFRSF14/HVEM primary antibodies
  • Description

    Rabbit monoclonal [EPR22122] to TNFRSF14/HVEM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TNFRSF14/HVEM aa 1-250. The exact sequence is proprietary.
    Database link: Q92956

  • Positive control

    • ICC/IF: HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct. Flow cyt: Human PBMCs and HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct.
  • General notes

     This product was previously labelled as TNFRSF14

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/400.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function

      Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
    • Tissue specificity

      Widely expressed, with the highest expression in lung, spleen and thymus.
    • Sequence similarities

      Contains 3 TNFR-Cys repeats.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • HVEML antibody
      • ATAR antibody
      • CD270 antibody
      • CD40 like protein precursor antibody
      • Herpes virus entry mediator A antibody
      • Herpesvirus entry mediator A antibody
      • Herpesvirus entry mediator antibody
      • Herpesvirus entry mediator ligand antibody
      • HveA antibody
      • HVEM antibody
      • HVEM L antibody
      • LIGHT antibody
      • LIGHTR antibody
      • TNFRSF14 antibody
      • TNFSF 14 antibody
      • TNR14_HUMAN antibody
      • TR2 antibody
      • Tumor necrosis factor receptor like gene2 antibody
      • Tumor necrosis factor receptor superfamily member 14 antibody
      • Tumor necrosis factor receptor superfamily member 14 precursor antibody
      • Tumor necrosis factor receptor-like 2 antibody
      see all

    Images

    • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with a Myc-tagged human TNFRSF14/HVEM expression construct. The nuclear counter stain is DAPI (blue). Cells are counter stained for Myc using an Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (ab223894) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    References

    ab224708 has not yet been referenced specifically in any publications.

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