Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22122] to TNFRSF14/HVEM
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-TNFRSF14/HVEM antibody [EPR22122]
See all TNFRSF14/HVEM primary antibodies
DescriptionRabbit monoclonal [EPR22122] to TNFRSF14/HVEM
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB
Species reactivityReacts with: Human
Recombinant fragment within Human TNFRSF14/HVEM aa 1-250. The exact sequence is proprietary.
Database link: Q92956
- ICC/IF: HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct. Flow cyt: Human PBMCs and HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct.
This product was previously labelled as TNFRSF14
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab224708 in the following tested applications.
FunctionReceptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
Tissue specificityWidely expressed, with the highest expression in lung, spleen and thymus.
Sequence similaritiesContains 3 TNFR-Cys repeats.
- Information by UniProt
- HVEML antibody
- ATAR antibody
- CD270 antibody
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with a Myc-tagged human TNFRSF14/HVEM expression construct. The nuclear counter stain is DAPI (blue). Cells are counter stained for Myc using an Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (ab223894) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224708 has not yet been referenced specifically in any publications.