Product nameAnti-TNFSF18/GITRL antibody [EPR20583]
See all TNFSF18/GITRL primary antibodies
DescriptionRabbit monoclonal [EPR20583] to TNFSF18/GITRL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TNFSF18/GITRL aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UNG2
- WB: HUVEC whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).|
FunctionCytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
Tissue specificityExpressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- Activation inducible TNF related ligand antibody
- Activation-inducible TNF-related ligand antibody
- AITR ligand antibody
All lanes : Anti-TNFSF18/GITRL antibody [EPR20583] (ab211037) at 1/5000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
TNFSF18 is constitutively expressed in HUVEC cells but is not expressed in monocytic cell line.
Negative control: THP-1 (PMID: 10037686).
ab211037 has not yet been referenced specifically in any publications.