Recombinant
RabMAb

Recombinant Anti-TNFSF18/GITRL antibody [EPR20583] (ab211037)

Overview

  • Product name

    Anti-TNFSF18/GITRL antibody [EPR20583]
    See all TNFSF18/GITRL primary antibodies
  • Description

    Rabbit monoclonal [EPR20583] to TNFSF18/GITRL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TNFSF18/GITRL aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UNG2

  • Positive control

    • WB: HUVEC whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
  • Tissue specificity

    Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation inducible TNF related ligand antibody
    • Activation-inducible TNF-related ligand antibody
    • AITR ligand antibody
    • AITRL antibody
    • GITR ligand antibody
    • GITRL antibody
    • Glucocorticoid induced TNF related ligand antibody
    • Glucocorticoid induced TNFR related protein ligand antibody
    • Glucocorticoid-induced TNF-related ligand antibody
    • hGITRL antibody
    • MGC138237 antibody
    • TL6 antibody
    • TNF18_HUMAN antibody
    • TNFSF18 antibody
    • TNLG2A antibody
    • Tumor necrosis factor (ligand) superfamily member 18 antibody
    • Tumor necrosis factor ligand 2A antibody
    • Tumor necrosis factor ligand superfamily member 18 antibody
    see all

Images

  • All lanes : Anti-TNFSF18/GITRL antibody [EPR20583] (ab211037) at 1/5000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNFSF18 is constitutively expressed in HUVEC cells but is not expressed in monocytic cell line.

    Negative control: THP-1 (PMID: 10037686).

References

ab211037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211037.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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