Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20583] to TNFSF18/GITRL
- Suitable for: WB
- Reacts with: Human
Product nameAnti-TNFSF18/GITRL antibody [EPR20583]
See all TNFSF18/GITRL primary antibodies
DescriptionRabbit monoclonal [EPR20583] to TNFSF18/GITRL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TNFSF18/GITRL aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UNG2
- WB: HUVEC whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).|
FunctionCytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
Tissue specificityExpressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- Activation inducible TNF related ligand antibody
- Activation-inducible TNF-related ligand antibody
- AITR ligand antibody
All lanes : Anti-TNFSF18/GITRL antibody [EPR20583] (ab211037) at 1/5000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
TNFSF18 is constitutively expressed in HUVEC cells but is not expressed in monocytic cell line.
Negative control: THP-1 (PMID: 10037686).
ab211037 has not yet been referenced specifically in any publications.