Product nameAnti-TNFSF18/GITRL antibody [EPR23451-119] - BSA and Azide free
See all TNFSF18/GITRL primary antibodies
DescriptionRabbit monoclonal [EPR23451-119] to TNFSF18/GITRL - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment within Human TNFSF18/GITRL aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UNG2
- FC: HUVEC cells.
ab272696 is the carrier-free version of ab254383. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab272696 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab272696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionCytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
Tissue specificityExpressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- Activation inducible TNF related ligand antibody
- Activation-inducible TNF-related ligand antibody
- AITR ligand antibody
Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) treated with 1ug/ml lipopolysaccharide for 24h (Red) / Untreated control (Green) labelling TNFSF18/GITRL with ab254383 at 1/500 dilution (Red) / (Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells (PMID: 10037686, 17449724).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254383).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272696 has not yet been referenced specifically in any publications.