Key features and details
- Rabbit polyclonal to TNIK
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TNIK antibody
See all TNIK primary antibodies
DescriptionRabbit polyclonal to TNIK
SpecificityBLAST analysis of the peptide immunogen showed no homology with other human proteins.
Tested applicationsSuitable for: IHC-P, ICCmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Xenopus laevis, Lizard, Monkey, Gorilla, Opossum, Common marmoset, Bat, Elephant
Synthetic peptide corresponding to Human TNIK (internal sequence). Synthetic peptide corresponding to 18 amino acids within TNIK (Q9UKE5, NM_015028, BAA25477.2).
- Human kidney tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
FunctionSerine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
Tissue specificityExpressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
Cellular localizationNucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
- Information by UniProt
- EC 22.214.171.124 antibody
- KIAA0551 antibody
- Tnik antibody
ab150739 has been referenced in 1 publication.
- Gal J et al. Detergent Insoluble Proteins and Inclusion Body-Like Structures Immunoreactive for PRKDC/DNA-PK/DNA-PKcs, FTL, NNT, and AIFM1 in the Amygdala of Cognitively Impaired Elderly Persons. J Neuropathol Exp Neurol 77:21-39 (2018). PubMed: 29186589