Anti-TNIP1 antibody (ab236984)
Key features and details
- Rabbit polyclonal to TNIP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TNIP1 antibody
See all TNIP1 primary antibodies -
Description
Rabbit polyclonal to TNIP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human TNIP1 aa 500 to the C-terminus.
Database link: Q15025-1 -
Positive control
- IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells. WB: Rat kidney tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 7 µg/ml. Predicted molecular weight: 72 kDa.
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
Use a concentration of 7 µg/ml. Predicted molecular weight: 72 kDa. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal (By similarity). Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. -
Tissue specificity
Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 10318 Human
- Entrez Gene: 363599 Rat
- Omim: 607714 Human
- SwissProt: Q15025 Human
- Unigene: 355141 Human
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Alternative names
- ABIN 1 antibody
- HIV-1 Nef-interacting protein antibody
- hVAN antibody
see all
Images
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Positive WB detected in: Rat kidney tissue.
All lanes: ab236984 antibody at 7μg/ml.
Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 72, 67, 65, 63, 62 kDa
Observed band size: 72 kDa -
Paraffin-embedded human skeletal muscle tissue stained for TNIP1 with ab236984 at 1/300 dilution in immunohistochemical analysis.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TNIP1 (green) using ab236984 at 1/100 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236984 has not yet been referenced specifically in any publications.