Key features and details
- Rabbit polyclonal to TNIP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TNIP2 antibody
See all TNIP2 primary antibodies
DescriptionRabbit polyclonal to TNIP2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 46-308 of Human TNIP2 (UniProt ID: Q8NFZ5).
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate; HeLa cells; Human SAS xenograft tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionInhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation.
Tissue specificityUbiquitously expressed in all tissues examined.
- Information by UniProt
- A20 binding inhibitor of NF kappaB activation 2 antibody
- A20-binding inhibitor of NF-kappa-B activation 2 antibody
- ABIN 2 antibody
All lanes : Anti-TNIP2 antibody (ab155513) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TNIP2 at 1/200 dilution. Lower lower image merged with DNA probe.
Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling TNIP2 at 1/500 dilution.
ab155513 has not yet been referenced specifically in any publications.