Key features and details
- Rabbit polyclonal to TNNC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TNNC1 antibody
See all TNNC1 primary antibodies
DescriptionRabbit polyclonal to TNNC1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Quail
Recombinant full length protein corresponding to Human TNNC1 aa 1-160.
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E
Database link: P63316
- WB: Mouse heart, lung and skeletal muscle tissue lysates; IHC: Human colon carcinoma ICC/ IF: U-2 OS cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 18 kDa.|
FunctionTroponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
Involvement in diseaseDefects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNC1 are the cause of cardiomyopathy familial hypertrophic type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Sequence similaritiesBelongs to the troponin C family.
Contains 4 EF-hand domains.
- Information by UniProt
- Cardiac troponin C antibody
- CMD1Z antibody
- CMH13 antibody
Paraffin-embedded human colon carcinoma tissue stained for TNNC1 using ab180732 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of U-2 OS cells stained for TNNC1 with ab180732. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-TNNC1 antibody (ab180732) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse skeletal muscle lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 18 kDa
Blocking buffer: 3% nonfat dry milk in TBST.
ab180732 has not yet been referenced specifically in any publications.