Overview

  • Product name

  • Description

    Rabbit polyclonal to TNNI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A peptide from near the N terminal residues of human TNNI1 protein (NM_003281).

  • Positive control

    • Skeletal muscle

Properties

  • Form

    Lyophilised:Reconstitute in 200 µl sterile H2O.
  • Storage instructions

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Peptide ELISA titer, 1:1,000-1:10,000.
    IHC-P: 1/100 - 1/500.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Tissue specificity

      Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
    • Sequence similarities

      Belongs to the troponin I family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2700018B22Rik antibody
      • AI747285 antibody
      • DKFZp451O223 antibody
      • si:dz182n13.3 antibody
      • slow skeletal muscle antibody
      • slow-twitch isoform antibody
      • SSTNI antibody
      • TNN1 antibody
      • Tnni1 antibody
      • TNNI1_HUMAN antibody
      • Troponin I antibody
      • Troponin I slow skeletal muscle antibody
      • Troponin I slow twitch isoform antibody
      • troponin I type 1 (skeletal, slow) antibody
      • Troponin I, slow isoform antibody
      see all

    Images

    • Immunohistochemical analysis of TNNI1 expression in formalin fixed, paraffin embedded skeletal muscle using ab85087 at a 1/100 dilution. Note cytoplasmic staining.

    References

    This product has been referenced in:

    • Li XF  et al. Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1. Exp Mol Med 50:115 (2018). Read more (PubMed: 30185781) »
    • Grajales L  et al. Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4. J Mol Cell Cardiol 53:382-91 (2012). ICC/IF ; Human . Read more (PubMed: 22709559) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab85087.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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