Overview

  • Product name

    Anti-TNNI1 antibody [EPR17120-11]
    See all TNNI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17120-11] to TNNI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human TNNI1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P19237

  • Positive control

    • WB: Human fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle tissue. IP: Human fetal heart whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17120-11
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/50.

Target

  • Function

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
  • Sequence similarities

    Belongs to the troponin I family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2700018B22Rik antibody
    • AI747285 antibody
    • DKFZp451O223 antibody
    • si:dz182n13.3 antibody
    • slow skeletal muscle antibody
    • slow-twitch isoform antibody
    • SSTNI antibody
    • TNN1 antibody
    • Tnni1 antibody
    • TNNI1_HUMAN antibody
    • Troponin I antibody
    • Troponin I slow skeletal muscle antibody
    • Troponin I slow twitch isoform antibody
    • troponin I type 1 (skeletal, slow) antibody
    • Troponin I, slow isoform antibody
    see all

Images

  • All lanes : Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution + Human skeletal muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on striated Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab203515 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203515 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10 µg (Input). Lane 2: ab203515 IP in Human fetal heart whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203515 in Human fetal heart whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab203515 has not yet been referenced specifically in any publications.

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