Anti-TNNI3K antibody (ab111140)
Key features and details
- Rabbit polyclonal to TNNI3K
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNNI3K antibody
See all TNNI3K primary antibodies -
Description
Rabbit polyclonal to TNNI3K -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from an internal region of Human TNNI3K.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111140 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in cardiac physiology. -
Tissue specificity
Highly expressed in both adult and fetal heart. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 10 ANK repeats.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 100526835 Human
- Entrez Gene: 51086 Human
- Entrez Gene: 295531 Rat
- SwissProt: Q59H18 Human
- SwissProt: Q7TQP6 Rat
- Unigene: 729320 Human
- Unigene: 3434 Rat
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Alternative names
- Cardiac ankyrin repeat kinase antibody
- cardiac muscle antibody
- Cardiac troponin I-interacting kinase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI3K antibody (ab111140)ab111140, at a 1/50 dilution, staining TNNI3K in paraffin embedded Human lung carcinoma tissue by Immunohistochemistry. Image on the right was from tissue treated with the synthetic peptide.
Datasheets and documents
References (2)
ab111140 has been referenced in 2 publications.
- Patterson M et al. Frequency of mononuclear diploid cardiomyocytes underlies natural variation in heart regeneration. Nat Genet 49:1346-1353 (2017). PubMed: 28783163
- Theis JL et al. TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . PubMed: 24925317
