Overview

  • Product name
    Anti-TNNI3K antibody
    See all TNNI3K primary antibodies
  • Description
    Rabbit polyclonal to TNNI3K
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal region of Human TNNI3K.

  • Positive control
    • Human lung carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111140 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 93 kDa. PubMed: 24925317
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in cardiac physiology.
  • Tissue specificity
    Highly expressed in both adult and fetal heart.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 10 ANK repeats.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Cardiac ankyrin repeat kinase antibody
    • cardiac muscle antibody
    • Cardiac troponin I-interacting kinase antibody
    • CARK antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnl antibody
    • MGC142099 antibody
    • MGC33828 antibody
    • RCM1 antibody
    • Serine/threonine protein kinase TNN13K antibody
    • Serine/threonine-protein kinase TNNI3K antibody
    • TNI3K_HUMAN antibody
    • TNNC1 antibody
    • TNNI3 interacting kinase antibody
    • TNNI3-interacting kinase antibody
    • Tnni3k antibody
    • Troponin 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI3K antibody (ab111140)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI3K antibody (ab111140)
    ab111140, at a 1/50 dilution, staining TNNI3K in paraffin embedded Human lung carcinoma tissue by Immunohistochemistry. Image on the right was from tissue treated with the synthetic peptide.

References

This product has been referenced in:
  • Theis JL  et al. TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24925317) »

See 1 Publication for this product

Publishing research using ab111140? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

immunogen range or sequence?
her protein has point mutation within kinase domain

The amino acid range of this immunogen is aa 200-250 of human Serine/threonine-protein kinase TNNI3K with Uniprot accession number Q59H18 (http://www.uniprot.org/uniprot/Q59H18). This antibody binds to the ANK5 and ANK6 repeats of isoforms 1, 2, 3, and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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