Product nameAnti-TNNI3K antibody
See all TNNI3K primary antibodies
DescriptionRabbit polyclonal to TNNI3K
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chimpanzee, Macaque monkey
Synthetic peptide corresponding to Human TNNI3K aa 300-400 conjugated to keyhole limpet haemocyanin.
Database link: Q59H18
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Mouse Heart; Rat Heart.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab136954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).|
FunctionMay play a role in cardiac physiology.
Tissue specificityHighly expressed in both adult and fetal heart.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 10 ANK repeats.
Contains 1 protein kinase domain.
Cellular localizationNucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
- Information by UniProt
- Cardiac ankyrin repeat kinase antibody
- cardiac muscle antibody
- Cardiac troponin I-interacting kinase antibody
All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab136954 has not yet been referenced specifically in any publications.