Anti-TNNI3K antibody (ab136954)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TNNI3K antibody
See all TNNI3K primary antibodies -
Description
Rabbit polyclonal to TNNI3K -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human TNNI3K aa 300-400 conjugated to keyhole limpet haemocyanin.
Database link: Q59H18 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Mouse Heart; Rat Heart.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab136954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa). |
Target
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Function
May play a role in cardiac physiology. -
Tissue specificity
Highly expressed in both adult and fetal heart. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 10 ANK repeats.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 100526835 Human
- Entrez Gene: 51086 Human
- Entrez Gene: 435766 Mouse
- Entrez Gene: 295531 Rat
- SwissProt: Q59H18 Human
- SwissProt: Q5GIG6 Mouse
- SwissProt: Q7TQP6 Rat
- Unigene: 729320 Human
see all -
Alternative names
- Cardiac ankyrin repeat kinase antibody
- cardiac muscle antibody
- Cardiac troponin I-interacting kinase antibody
see all
Images
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Western blot - Anti-TNNI3K antibody (ab136954)All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
References
ab136954 has not yet been referenced specifically in any publications.
