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Anti-TNNI3K antibody (ab136954)

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Western blot - Anti-TNNI3K antibody (ab136954)

    Key features and details

    • Rabbit polyclonal to TNNI3K
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human TNNI3K protein (ab162409)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-TNNI3K antibody
      See all TNNI3K primary antibodies
    • Description

      Rabbit polyclonal to TNNI3K
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Rabbit, Chimpanzee, Macaque monkey
    • Immunogen

      Synthetic peptide corresponding to Human TNNI3K aa 300-400 conjugated to keyhole limpet haemocyanin.
      Database link: Q59H18

    • Positive control

      • This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Mouse Heart; Rat Heart.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Heart
      • Cardiac metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human TNNI3K protein (ab162409)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab136954 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).

    Target

    • Function

      May play a role in cardiac physiology.
    • Tissue specificity

      Highly expressed in both adult and fetal heart.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 10 ANK repeats.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Cellular localization

      Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
    • Target information above from: UniProt accession Q59H18 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 100526835 Human
      • Entrez Gene: 51086 Human
      • Entrez Gene: 435766 Mouse
      • Entrez Gene: 295531 Rat
      • SwissProt: Q59H18 Human
      • SwissProt: Q5GIG6 Mouse
      • SwissProt: Q7TQP6 Rat
      • Unigene: 729320 Human
      • Unigene: 210428 Mouse
      • Unigene: 3434 Rat
      see all
    • Alternative names

      • Cardiac ankyrin repeat kinase antibody
      • cardiac muscle antibody
      • Cardiac troponin I-interacting kinase antibody
      • CARK antibody
      • CMD1FF antibody
      • CMD2A antibody
      • CMH7 antibody
      • cTnl antibody
      • MGC142099 antibody
      • MGC33828 antibody
      • RCM1 antibody
      • Serine/threonine protein kinase TNN13K antibody
      • Serine/threonine-protein kinase TNNI3K antibody
      • TNI3K_HUMAN antibody
      • TNNC1 antibody
      • TNNI3 interacting kinase antibody
      • TNNI3-interacting kinase antibody
      • Tnni3k antibody
      • Troponin 1 antibody
      see all

    Images

    • Western blot - Anti-TNNI3K antibody (ab136954)
      Western blot - Anti-TNNI3K antibody (ab136954)
      All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
      Lane 3 : Heart (Mouse) Tissue Lysate
      Lane 4 : Heart (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 93 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab136954? Please let us know so that we can cite the reference in this datasheet.

    ab136954 has not yet been referenced specifically in any publications.

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