Overview

  • Product name

  • Description

    Rabbit polyclonal to TNNI3K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human TNNI3K aa 300-400 conjugated to keyhole limpet haemocyanin.
    Database link: Q59H18

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Mouse Heart; Rat Heart.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    May play a role in cardiac physiology.
  • Tissue specificity

    Highly expressed in both adult and fetal heart.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 10 ANK repeats.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac ankyrin repeat kinase antibody
    • cardiac muscle antibody
    • Cardiac troponin I-interacting kinase antibody
    • CARK antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnl antibody
    • MGC142099 antibody
    • MGC33828 antibody
    • RCM1 antibody
    • Serine/threonine protein kinase TNN13K antibody
    • Serine/threonine-protein kinase TNNI3K antibody
    • TNI3K_HUMAN antibody
    • TNNC1 antibody
    • TNNI3 interacting kinase antibody
    • TNNI3-interacting kinase antibody
    • Tnni3k antibody
    • Troponin 1 antibody
    see all

Images

  • All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 93 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab136954 has not yet been referenced specifically in any publications.

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