Key features and details
- Goat polyclonal to TNNT3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TNNT3 antibody - C-terminal
See all TNNT3 primary antibodies
DescriptionGoat polyclonal to TNNT3 - C-terminal
Specificityab175058 is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human, Mouse and Rat skeletal muscle lysates
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab175058 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab175058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.
1 hour primary incubation is recommended for this product.
FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Tissue specificityIn fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
Involvement in diseaseDefects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
Sequence similaritiesBelongs to the troponin T family.
- Information by UniProt
- AMCD2B antibody
- Beta TnTF antibody
- Beta-TnTF antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling TNNT3 with ab175058 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
All lanes : Anti-TNNT3 antibody - C-terminal (ab175058) at 0.01 µg/ml
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse skeletal muscle lysate
Lane 3 : Rat skeletal muscle lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 32 kDa
ab175058 has not yet been referenced specifically in any publications.