Overview

  • Product name

  • Description

    Rabbit polyclonal to TNP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human TNP1 aa 1 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104948)

  • Positive control

    • This antibody gave a positive signal in Human normal testis FFPE section.
  • General notes

    Previously labelled as Transition Protein 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab73135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References

This product has been referenced in:

  • Zhou H  et al. The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation. Genesis 57:e23295 (2019). Read more (PubMed: 31001916) »
  • Han H  et al. Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny. Asian-Australas J Anim Sci 29:793-800 (2016). IF . Read more (PubMed: 26954128) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Bouin's solution

Abcam user community

Verified customer

Submitted May 06 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Testes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (NuPage 4-12% Bis-Tris)
Loading amount
1 µg
Specification
Testes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 08 2013

Answer

I am very pleased to hear you would like to accept our offer and test ab73135 in Western Blot. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Western Blot. The code is inactive until included in your submitted Abreview so be sure to add it to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

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